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Establishment of an efficient callus induction and plant regeneration system in Pakistani wheat (Triticum aestivum) cultivars
Rashid, Umer; Ali, Shaukat; Ali, Ghulam Muhammad; Ayub, Najma; Masood, M. Shahid.
  • Rashid, Umer; Quaid-i-Azam University. Faculty of Biological Sciences. Deparment of Microbiology and Biotechnology. Islamabad. PK
  • Ali, Shaukat; National Agriculture Research Center. Plant Biotechnology Program. Islamabad. PK
  • Ali, Ghulam Muhammad; National Agriculture Research Center. Plant Biotechnology Program. Islamabad. PK
  • Ayub, Najma; Quaid-i-Azam University. Faculty of Biological Sciences. Deparment of Microbiology and Biotechnology. Islamabad. PK
  • Masood, M. Shahid; National Agriculture Research Center. Plant Biotechnology Program. Islamabad. PK
Electron. j. biotechnol ; 12(3): 4-5, July 2009. ilus, tab
Article in English | LILACS | ID: lil-551882
ABSTRACT
Four commercially grown wheat varieties of Pakistan, namely Inqilab-91, Chakwal-97, Tatara and Manthar were used for this investigation. For callus induction different concentrations of 2,4-Dichlorophenoxyaceticacid (2,4-D) along with 0.1 mg/L of Kinetin were evaluated. For regeneration initially different concentrations of Indole-3-Acetic Acid (IAA) and 6-BenzylAminoPurine (BAP) were tested. Best hormone combinations were further subjected to Kinetin and 6-ã-ã-dimethylallylaminopurine (2iP). For Inqilab-91, Chakwal-97 and Manthar, 3 mg/L of 2,4-D was found optimum, which induced 83.25 percent, 77.75 percent and 95.20 percent of embryogenic calli, respectively. Maximum callus induction (97.18 percent) was observed in Tatara when 2 mg/L of 2,4-D was used. As regard to regeneration, Inqilab-91, Chakwal-97 and Manthar showed maximum regeneration on media containing 0.1 mg/L IAA, 0.4 mg/L Kinetin and 0.5 mg/L 2iP, regenerating 87.25 percent, 81.75 percent and 68.75 percent respectively. For Tatara maximum regeneration of 12.25 percent was obtained on 0.1 mg/L IAA and 2 mg/L of BAP. Presently optimized regeneration method holds promise for facilitating the deployment of agronomical important trait through genetic transformation for the improvement of this important food crop.
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Full text: Available Index: LILACS (Americas) Main subject: Triticum / Embryonic Development Country/Region as subject: Asia Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2009 Type: Article Affiliation country: Pakistan Institution/Affiliation country: National Agriculture Research Center/PK / Quaid-i-Azam University/PK

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Full text: Available Index: LILACS (Americas) Main subject: Triticum / Embryonic Development Country/Region as subject: Asia Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2009 Type: Article Affiliation country: Pakistan Institution/Affiliation country: National Agriculture Research Center/PK / Quaid-i-Azam University/PK