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Ischemia-reperfusion histopathology alterations of the rabbit intestinal wall with and without exclusion of the collateral mesenteric circulation supply / Alterações histopatológicas da parede intestinal de coelhos na isquemia-reperfusão com e sem exclusão da circulação mesentérica colateral
Gomes, Otoni Moreira; Brasileiro Filho, Geraldo; Porto, Luiz Alberto Bomjardim; Prata, Pedro Henrique de Lima; Paixão, Rafael de Mattos.
  • Gomes, Otoni Moreira; Minas Gerais Federal University. School of Medicine. Department of Surgery. BR
  • Brasileiro Filho, Geraldo; Minas Gerais Federal University. School of Medicine. Department of Pathology. BR
  • Porto, Luiz Alberto Bomjardim; Minas Gerais Federal University. School of Medicine. BR
  • Prata, Pedro Henrique de Lima; Minas Gerais Federal University. School of Medicine. BR
  • Paixão, Rafael de Mattos; Minas Gerais Federal University. School of Medicine. Surgery Department. Surgical Technique Division. BR
Acta cir. bras ; 25(4): 318-321, July-Aug. 2010. ilus, graf, tab
Article in English | LILACS | ID: lil-553236
ABSTRACT

PURPOSE:

To evaluate the histopathology alterations of the intestinal mucosa of rabbits submitted to different times of mesenteric artery ischemia and reperfusion with and without celiac artery collateral circulation supply.

METHODS:

Two groups of eight male New Zealand white rabbits (weight 2.2-3.5 kg) were used in this study. In the Group 1 animals, the proximal mesenteric artery was occluded for 60 min with an atraumatic vascular clamp, followed by reperfusion for 60 min. In the Group 2 animals the small bowel and mesentery were cut 30cm and 60cm far from the gastroduodenal pyloric transition before the proximal mesenteric artery occlusion. Small bowel biopsies were obtained before ischemia (control), after 30 min and 60 min of mesenteric ischemia and at 30 and 60 min. of mesenteric artery reperfusion.

RESULTS:

In the Group I animals, the followings histopathology grade results were observed t1, mean 0.4 + 0.29; t2, mean 1.9 ± 0.38; t3, 1.9 ± 0.33; t4, 1.2 ± 0.36 and t5, 1.2 ± 0.32. Differences between t0 and t2 and between t3 and t4 were statistically significant (p<0.05). Differences between t2 and t3 and t4 and t5 were not significant (p>0.5). In the Group II animals, it was observed t1, mean 1.6 ± 0.33; t2, 2.4 ± 0.36; t3, 3.0 ± 0.35; t4 3.4 ± 0.31; t5, 3 ± 031. Differences between t0 and t1, t1 and t2, and t2 and t3 were significant (p<0.05). Differences between histopathology grades results of samples t1 to t5 in Group 1 and 2 were statistically significant (p<0.5).

CONCLUSION:

Microscopic examination of the biopsies revealed significant evidence of worse small bowel wall ischemia-reperfusion lesions by exclusion of the celiac artery collateral circulation supply.
RESUMO

OBJETIVO:

Avaliar as alterações histopatológicas da mucosa intestinal de coelhos submetidos à isquemia-reperfusão com e sem exclusão da circulação mesentérica colateral.

MÉTODOS:

Foram estudados dois grupos de oito coelhos Nova Zelândia machos com pesos variáveis entre 2,2 e 3,5 kg de peso corpóreo. Nos animais do Grupo 1, a artéria mesentérica proximal foi ocluida por pinçamento atraumático durante 60 min, seguido de reperfusão por 60 min. No Grupo 2 o intestino delgado e o mesentério foram seccionados 30 cm e 60 após a transição pilórica gastroduodenal antes da oclusão da artéria mesentérica cranial. Biópsias da parede intestinal foram obtidas antes da isquemia (controle), após 30 e 60 min. de isquemia.

RESULTADOS:

No Grupo I foram observados os seguintes graus de lesões t1,média de 0.4 + 0.29; t2, média 1.9 ± 0.38; t3, 1.9 ± 0.33; t4, 1.2 ± 0.36 e t5, 1.2 ± 0.32. As diferenças entre t0 e t2 e entre t3 e t4 foram significantes (p<0.05). As diferenças entre t2 e t3 e t4 e t5 não foram significantes (p>0.5). No Group II observou-se t1, média de 1.6 ± 0.33; t2, 2.4 ± 0.36; t3, 3.0 ± 0.35; t4 3.4 ± 0.31; t5, 3 ± 031. As diferenças entre t0 e t1, t1 e t2, e t2 e t3 foram significantes (p<0.05). As diferenças entre os resultados histopatológicos das biopsies de t1 a t5 dos Grupos 1 e 2 foram significantes (p<0.5).

CONCLUSÃO:

A exclusão da circulação mesentérica colateral agravou significantemente a degeneração histopatológica na isquemia-reperfusão da parede intestinal.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Splanchnic Circulation / Reperfusion Injury / Collateral Circulation / Intestine, Small / Mesenteric Arteries / Mesenteric Vascular Occlusion Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2010 Type: Article Affiliation country: Brazil Institution/Affiliation country: Minas Gerais Federal University/BR

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Full text: Available Index: LILACS (Americas) Main subject: Splanchnic Circulation / Reperfusion Injury / Collateral Circulation / Intestine, Small / Mesenteric Arteries / Mesenteric Vascular Occlusion Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2010 Type: Article Affiliation country: Brazil Institution/Affiliation country: Minas Gerais Federal University/BR