Rapid and inexpensive analysis of genetic variability in Arapaima gigas by PCR multiplex panel of eight microsatellites
Genet. mol. res. (Online)
;
7(1): 29-32, Jan. 2008. tab
Article
in English
| LILACS
| ID: lil-553767
ABSTRACT
The aim of the present study was the development of a multiplex genotyping panel of eight microsatellite markers of Arapaima gigas, previously described. Specific primer pairs were developed, each one of them marked with either FAM-6, HEX or NED. The amplification conditions using the new primers were standardized for a single reaction. The results obtained demonstrate high heterozygosity (average of 0.69) in a Lower Amazon population. The multiplex system described can thus be considered a fast, efficient and inexpensive method for the investigation of genetic variability in Arapaima populations.
Full text:
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Index:
LILACS (Americas)
Main subject:
Genetic Variation
/
Polymerase Chain Reaction
/
Microsatellite Repeats
/
Fishes
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Genet. mol. res. (Online)
Journal subject:
Molecular Biology
/
Genetics
Year:
2008
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal do Pará/BR
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