Genetic diversity in the Pantaneiro horse breed assessed using microsatellite DNA markers
Genet. mol. res. (Online)
;
7(1): 261-270, Jan. 2008. ilus, mapas, tab, graf
Article
in English
| LILACS
| ID: lil-553793
ABSTRACT
The genetic variability for a sample of 227 animals from three populations of Pantaneiro horses was estimated using data from 10 microsatellite loci. The number of alleles and the proportion of heterozygosity indicated high variability. A total of 91 alleles were found, with a significantly high mean number of alleles. The mean polymorphic information content was 0.7 and the paternity exclusion probability was 99.3%. The inbreeding coefficient (Fis) was low for the three populations Ipiranga (FIS = 0.147), Nova Esperança (Fis = 0.094) and Promissão (Fis = 0.108). Genetic differentiation among all three populations was low (FST = 0.008 to 0.064). Three methods were used to test for a recent bottleneck effect. The graphical method and the Wilcoxon test using the stepwise mutation model showed no bottleneck pattern for any of the populations. The test by two-phase mutation model showed genetic signatures of bottleneck for Ipiranga and Promissão. When we consider standard deviation value for Nova Esperança, the M-statistic detected a bottleneck pattern, but this result could be explained by a sample size effect. Therefore, there is no immediate cause for concern regarding loss of variation within the breed.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Genetic Variation
/
Microsatellite Repeats
/
Horses
Type of study:
Prognostic study
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Genet. mol. res. (Online)
Journal subject:
Molecular Biology
/
Genetics
Year:
2008
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal do Rio Grande do Sul/BR
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