Your browser doesn't support javascript.
loading
Lipoic acid increases glutathione peroxidase, Na+, K+-ATPase and acetylcholinesterase activities in rat hippocampus after pilocarpine-induced seizures? / O ácido lipóico aumenta as atividades da glutationa peroxidase, da Na+, K+-ATPase e da acetilcolinesterase no hipocampo de ratos após convulsões induzidas por pilocarpina?
Souza, Geane Felix de; Saldanha, Gláucio Barros; Freitas, Rivelilson Mendes de.
  • Souza, Geane Felix de; Clinical Pathology Laboratory. Fortaleza. BR
  • Saldanha, Gláucio Barros; Clinical Pathology Laboratory. Fortaleza. BR
  • Freitas, Rivelilson Mendes de; Federal University of Piaui. Laboratory of Experimental Research in Biological Sciences. Picos. BR
Arq. neuropsiquiatr ; 68(4): 586-591, Aug. 2010. graf, tab
Article in English | LILACS | ID: lil-555239
Responsible library: BR1.1
ABSTRACT
In the present study we investigated the effects of lipoic acid (LA) on acetylcholinesterase (AChE), glutathione peroxidase (GPx) and Na+, K+-ATPase activities in rat hippocampus during seizures. Wistar rats were treated with 0.9 percent saline (i.p., control group), lipoic acid (20 mg/kg, i.p., LA group), pilocarpine (400 mg/kg, i.p., P400 group), and the association of pilocarpine (400 mg/kg, i.p.) plus LA (20 mg/kg, i.p.), 30 min before of administration of P400 (LA plus P400 group). After the treatments all groups were observed for 1 h. In P400 group, there was a significant increase in GPx activity as well as a decrease in AChE and Na+, K+-ATPase activities after seizures. In turn, LA plus P400 abolished the appearance of seizures and reversed the decreased in AChE and Na+, K+-ATPase activities produced by seizures, when compared to the P400 seizing group. The results from the present study demonstrate that preadministration of LA abolished seizure episodes induced by pilocarpine in rat, probably by increasing AChE and Na+, K+-ATPase activities in rat hippocampus.
RESUMO
No presente estudo nós investigamos os efeitos do ácido lipóico (AL) sobre as atividades da acetilcolinesterase (AChE), da glutationa peroxidase (GPx) e da Na+, K+-ATPase no hipocampo de ratos durante crises convulsivas. Ratos Wistar foram tratados com solução salina a 0,9 por cento (i.p., grupo controle), ácido lipóico (20 mg/kg, i.p., grupo AL), pilocarpina (400 mg/kg, i.p., grupo P400), e a associação de AL (20 mg/kg, i.p.) com a pilocarpina (400 mg/kg, i.p.), 30 min antes da administração de pilocarpina (grupo AL + P400). Após os tratamentos todos os grupos foram observados durante 1 h. No grupo P400, houve um aumento significativo na atividade da GPx, assim como uma diminuição das atividades da AChE e Na+, K+-ATPase. Por sua vez, o pré-tratamento com AL aboliu o aparecimento de convulsões e reverteu a diminuição das atividades da AChE e da Na+, K+-ATPase causadas pelas convulsões, quando comparada com o grupo P400 sozinho. Os resultados do estudo demonstram que o pré-tratamento com AL aboliu os episódios de convulsão induzido pela pilocarpina em ratos, provavelmente por meio do aumento das atividades das enzimas AChE e Na+, K+-ATPase no hipocampo de ratos.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Acetylcholinesterase / Seizures / Thioctic Acid / Sodium-Potassium-Exchanging ATPase / Glutathione Peroxidase / Hippocampus / Antioxidants Limits: Animals Language: English Journal: Arq. neuropsiquiatr Journal subject: Neurology / Psychiatry Year: 2010 Type: Article Affiliation country: Brazil Institution/Affiliation country: Clinical Pathology Laboratory/BR / Federal University of Piaui/BR
Full text: Available Index: LILACS (Americas) Main subject: Acetylcholinesterase / Seizures / Thioctic Acid / Sodium-Potassium-Exchanging ATPase / Glutathione Peroxidase / Hippocampus / Antioxidants Limits: Animals Language: English Journal: Arq. neuropsiquiatr Journal subject: Neurology / Psychiatry Year: 2010 Type: Article Affiliation country: Brazil Institution/Affiliation country: Clinical Pathology Laboratory/BR / Federal University of Piaui/BR