Cloning, mapping and molecular characterization of porcine progesterone receptor membrane component 2 (PGRMC2) gene
Genet. mol. biol
;
33(3): 471-474, 2010. ilus, tab
Article
in English
| LILACS
| ID: lil-555822
ABSTRACT
Progesterone plays an important role in sow reproduction by stimulating classic genomic pathways via nuclear receptors and non-genomic pathways via membrane receptors such a progesterone receptor membrane component 2 (PGRMC2). In this work, we used radiation hybrid mapping to assign PGRMC2 to pig chromosome 8 and observed that this receptor has two transcripts in pigs. The full-length cDNA of the large transcript is 1858 bp long and contains a 669-bp open reading frame (ORF) encoding a protein of 223 amino acids. The shorter transcript encodes a protein of 170 amino acids. The porcine PGRMC2 gene consists of three exons 446 bp, 156 bp and 1259 bp in length. The promoter sequence is GC-rich and lacks a typical TATA box. Several putative cis-regulatory DNA motifs were identified in the 208-bp upstream genomic region. Five single nucleotide polymorphisms (SNPs) were detected in introns* and the 3' UTR. RT-PCR indicated that the PGRMC2 gene is expressed ubiquitously in all pig tissues examined.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Swine
/
Receptors, Progesterone
Limits:
Animals
/
Humans
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
2010
Type:
Article
Affiliation country:
China
/
United kingdom
Institution/Affiliation country:
Ministry of Agriculture of China/CN
/
University of Cambridge/GB
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