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Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection
Eickhoff, Christopher S; Giddings, Olivia K; Yoshida, Nobuko; Hoft, Daniel F.
  • Eickhoff, Christopher S; s.af
  • Giddings, Olivia K; s.af
  • Yoshida, Nobuko; Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo. BR
  • Hoft, Daniel F; Saint Louis University. Department of Molecular Microbiology. US
Mem. Inst. Oswaldo Cruz ; 105(5): 687-691, Aug. 2010. graf
Article in English | LILACS | ID: lil-557231
ABSTRACT
The potential use of the Trypanosoma cruzi metacyclic trypomastigote (MT) stage-specific molecule glycoprotein-82 (gp82) as a vaccine target has not been fully explored. We show that the opsonization of T. cruzi MT with gp82-specific antibody prior to mucosal challenge significantly reduces parasite infectivity. In addition, we investigated the immune responses as well as the systemic and mucosal protective immunity induced by intranasal CpG-adjuvanted gp82 vaccination. Spleen cells from mice immunized with CpG-gp82 proliferated and secreted IFN-γ in a dose-dependent manner in response to in vitro stimulation with gp82 and parasite lysate. More importantly, these CpG-gp82-immunized mice were significantly protected from a biologically relevant oral parasite challenge.
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Full text: Available Index: LILACS (Americas) Main subject: Trypanosoma cruzi / Protozoan Proteins / Variant Surface Glycoproteins, Trypanosoma / Protozoan Vaccines / Chagas Disease Limits: Animals Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2010 Type: Article Affiliation country: Brazil / United States Institution/Affiliation country: Saint Louis University/US / Universidade Federal de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Trypanosoma cruzi / Protozoan Proteins / Variant Surface Glycoproteins, Trypanosoma / Protozoan Vaccines / Chagas Disease Limits: Animals Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2010 Type: Article Affiliation country: Brazil / United States Institution/Affiliation country: Saint Louis University/US / Universidade Federal de São Paulo/BR