Evaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patients
Mem. Inst. Oswaldo Cruz
;
105(5): 722-728, Aug. 2010. graf, tab
Article
in English
| LILACS
| ID: lil-557238
ABSTRACT
To evaluate commercial Lionex TB together with four antigens of Mycobacterium tuberculosis (MPT-64, MT10.3, 16 kDa and 38 kDa) for IgG and IgA cerebrospinal fluid (CSF) detection in the diagnosis of tuberculosis meningitis (TBM) with CSF negative acid-fast bacilli staining, 19 cases of TBM, 64 cases of other infectious meningoencephalitis and 73 cases of other neurological disorders were tested by enzyme linked immunosorbent assay. IgA-MPT-64 and IgG Lionex showed the highest sensitivities, specificities, positive predictive value and negative predictive value (63.2 percent, 47.4 percent; 95 percent, 93.7 percent; 40 percent, 98 percent and 28.4 percent, 97.1 percent, respectively). However, while grey zone was 12.7 percent and 6 percent, respectively, lowering sensitivity but maintains high specificity (> 95 percent). High protein concentration in CSF was associated with antibody positivity CSF/HIV+ which did not influence the sensitivity of both tests. To our knowledge, this is the first description of IgA-MPT-64 and IgG Lionex antibodies in CSF-TBM and, although there is good specificity, adjustments are needed based on antigen composition to enhance sensitivity.
Full text:
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Index:
LILACS (Americas)
Main subject:
Tuberculosis, Meningeal
/
Immunoglobulin A
/
Immunoglobulin G
/
Antibodies, Bacterial
/
Mycobacterium tuberculosis
/
Antigens, Bacterial
Type of study:
Diagnostic study
/
Evaluation studies
Limits:
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2010
Type:
Article
/
Project document
Affiliation country:
Brazil
/
Germany
Institution/Affiliation country:
Lionex Diagnostics and Therapeutics/DE
/
Universidade Federal do Rio de Janeiro/BR
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