Characterization of cellulolytic activities of Bjerkandera adusta and Pycnoporus sanguineus on solid wheat straw medium / Caracterización de las actividades celulolíticas de Bjerkandera adusta y Pycnoporus sanguineus en un medio sólido de paja de trigo
Electron. j. biotechnol
;
12(4): 5-6, Oct. 2009. ilus, tab
Article
in English
| LILACS
| ID: lil-558548
ABSTRACT
Cellulolytic properties of two white rot fungi, Bjerkandera adusta and Pycnoporus sanguineus, cultivated on wheat straw agar medium, were characterized and compared. Optimal growing parameters for maximum enzyme production for both fungi were wheat straw medium pH 5 and 28ºC. B. adusta showed, on the 6th day of culture, carboxymethylcellulose (CMC)ase activity levels 1.6 times higher than maximal P. sanguineus activity, achieved on the 8th day. B. adusta supernatants also displayed higher activity levels towards xylan (3.6-fold) compared to those of P. sanguineus. However, enzymes from P. sanguineus were more robust resisting one hour incubation at high temperatures (up to 80ºC), and exhibiting activity and stability in pH range from 2 to 8. Cellulolytic activities, with molecular masses ranging from 25 to 90 kDa, from the two species were detected in zymograms.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Triticum
/
Cellulose
/
Enzyme Activation
/
Fungi
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2009
Type:
Article
/
Project document
Affiliation country:
Mexico
Institution/Affiliation country:
Universidad Autónoma del Estado de Morelos/MX
/
Universidad Nacional Autónoma de México/MX
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