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Analysis of nucleus activity in malpighian tubules of Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) larvae by critical electrolyte concentration
Fermino, Fábio; Conte, Hélio; Falco, José R P.
  • Fermino, Fábio; s.af
  • Conte, Hélio; s.af
  • Falco, José R P; s.af
Neotrop. entomol ; 39(4): 568-571, July-Aug. 2010.
Article in English | LILACS | ID: lil-558843
Responsible library: BR1.1
ABSTRACT
Diatraea saccharalis (Fabricius) has cryptonephridial type Malphigian tubules (MT). This type of MT is characterized by the penetration of the distal part of the MT into the external walls of the rectum, which is usually lined with a perinephral membrane. The MT is divided into three differentiated regions proximal, middle and distal. In this study, our objective was to compare the nuclear activities of each one of the three regions of the D. saccharalis MT by using a nuclear basophilic technique and critical electrolyte concentration with a toluidine blue stain at pH 4.0. This method allows differentiation of DNA/protein complexes in in situ and in vitro chromatin. MT chromatin structure in D. saccharalis is variable. Fifth instars have a more decondensed chromatin than fourth instars. The distal tubule region was the most decondensed region of the MT. Our data show an elevated genetic activity of the MT in the pre-metamorphosis period. The distal region of the MT has the highest observed activity, which may be associated with the re-absorption of useful components and the excretion of waste materials.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Electrolytes / Lepidoptera / Malpighian Tubules Limits: Animals Language: English Journal: Neotrop. entomol Journal subject: Biology / ZOOLOGIA Year: 2010 Type: Article
Full text: Available Index: LILACS (Americas) Main subject: Electrolytes / Lepidoptera / Malpighian Tubules Limits: Animals Language: English Journal: Neotrop. entomol Journal subject: Biology / ZOOLOGIA Year: 2010 Type: Article