Development of potency assays for a plasmid containing vascular endothelial growth factor 2 / Desarrollo de la potencia de los ensayos para un plásmido que contiene factor 2 de crecimiento endotelial vascular
Electron. j. biotechnol
;
13(1): 1-2, Jan. 2010. ilus, tab
Article
in English
| LILACS
| ID: lil-559584
ABSTRACT
We have developed analytical methods to measure the biological functions of pVGI.1(VEGF2), a naked plasmid DNA product containing vascular endothelial growth factor 2 used in clinical trials for coronary artery diseases (CAD) and peripheral artery diseases (PAD). After being injected into muscles, vascular endothelial growth factor 2 (VEGF-2), presumably expressed in muscle tissues, binds to the endothelial cell receptors VEGFR2 or VEGFR3, triggering the downstream responses including cell proliferation and vascularization. As it is important to make sure clinical material is biological active, we developed a quantitative assay first to measure the receptor binding activity of the pVGI.1(VEGF2) gene product expressed by the transfected host cells, and then a qualitative assay to confirm the cell proliferation promoting activity of the expressed protein. In both assays the signals were plotted directly against input DNA concentrations used to transfect the host cells. We confirmed specificity for both assays. In addition, we demonstrated acceptable levels of spike recovery (86.7-116 percent), precision (largest relative standard deviation (RSD)=19.3 percent), linearity and range (60-140 percent relative potency, 15 - 35 ug/mL) for the quantitative assay. We intend to use the potency assays for routine lot release and stability studies.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Plasmids
/
Vascular Endothelial Growth Factor A
Type of study:
Diagnostic study
/
Evaluation studies
/
Qualitative research
Limits:
Adult
/
Animals
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2010
Type:
Article
Affiliation country:
United States
Institution/Affiliation country:
Corautus Genetics Inc/US
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