Enhancement of wanlongmycin production by nitrogen ion beam implantation / Mejora de la producción de wanlongmycin por implantación de haces iónicos de nitrógeno
Electron. j. biotechnol
;
13(1): 3-4, Jan. 2010. ilus, tab
Article
in English
| LILACS
| ID: lil-559586
ABSTRACT
In an attempt to obtain an industrial strain with higher yield of wanlongmycin, the wild strain Streptomyces griseovariabilis GAAS2507 was mutated by a novel mutagen, nitrogen ion beam with energy of 20 kilo electron volts (KeV) and dose ranging from 7.80 x 10(14) to 2.86 x 10(15) ions/cm². One mutant strain WN939 was obtained. Its yield of wanlongmycin reached 271.24 µg/mL, which was 82.10 percent higher than that of the wild strain. The mutant strain WN939 was relatively stable for the production of wanlongmycin through six successive transfers of cultures and a repeat fermentation in a 30 L fermentor. In addition, the mutant strains were investigated and divided into five types by their colony phenotypes and production of wanlongmycin. Among them, three types mutant strains exhibited positive mutation, while the other two types mutant strains exhibited negative mutation.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Oomycetes
/
Streptomyces
/
Mutagenesis
/
Mutagens
/
Mutation
Limits:
Animals
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2010
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
Guangdong Academy of Agricultural Science/CN
/
South China Agricultural University/CN
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