Urinary proteomics evaluation in interstitial cystitis/painful bladder syndrome: a pilot study
Int. braz. j. urol
;
36(4): 464-479, July-Aug. 2010. ilus, graf, tab
Article
in English
| LILACS
| ID: lil-562113
ABSTRACT
PURPOSE:
Interstitial cystitis/painful bladder syndrome (IC/PBS) is characterized by chronic pain, pressure and discomfort felt in the pelvis or bladder. An in-depth shotgun proteomics study was carried out to profile the urinary proteome of women with IC/PBS to identify possible specific proteins and networks associated with IC/PBS. MATERIALS ANDMETHODS:
Urine samples from ten female IC/PBS patients and ten female asymptomatic, healthy control subjects were analyzed in quadruplicate by liquid chromatography-tandem mass spectrometry (LC-MS/MS) on a hybrid linear ion trap-orbitrap mass spectrometer. Gas-phase fractionation (GPF) was used to enhance protein identification. Differences in protein quantity were determined by peptide spectral counting.RESULTS:
a-1B-glycoprotein (A1BG) and orosomucoid-1 (ORM1) were detected in all IC/PBS patients, and = 60 percent of these patients had elevated expression of these two proteins compared to control subjects. Transthyretin (TTR) and hemopexin (HPX) were detected in all control individuals, but = 60 percent of the IC/PBS patients had decreased expression levels of these two proteins. Enrichment functional analysis showed cell adhesion and response to stimuli were down-regulated whereas response to inflammation, wounding, and tissue degradation were up-regulated in IC/PBS. Activation of neurophysiological processes in synaptic inhibition, and lack of DNA damage repair may also be key components of IC/PBS.CONCLUSION:
There are qualitative and quantitative differences between the urinary proteomes of women with and without IC/PBS. We identified a number of proteins as well as pathways/networks that might contribute to the pathology of IC/PBS or result from perturbations induced by this condition.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Urine
/
Biomarkers
/
Proteins
/
Cystitis, Interstitial
/
Proteomics
Type of study:
Qualitative research
Limits:
Female
/
Humans
Language:
English
Journal:
Int. braz. j. urol
Journal subject:
Urology
Year:
2010
Type:
Article
/
Project document
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