Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol
Braz. arch. biol. technol
;
53(5): 1051-1056, Sept.-Oct. 2010. ilus, tab
Article
in English
| LILACS
| ID: lil-564081
ABSTRACT
The aim of this work was to study the isolation of glucose oxidase (GOx) from Aspergillus niger in aqueous two phase system consisting of PEG 7500 (150g l-1), potassium phosphate (175 g l-1, K2HPO4 +KH2PO4) and glucose (10 gl-1), the enzyme was partitioned in polymer phase. By sequential extraction GOx (69.2 percent) was recovered in polymer phase by 11.8 fold purification, giving a yield of 129U mg protein-¹.
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Index:
LILACS (Americas)
Language:
English
Journal:
Braz. arch. biol. technol
Journal subject:
Biology
Year:
2010
Type:
Article
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