Increased frequency of anti-retina antibodies in asymptomatic patients with chronic t. gondii infection
Clinics
;
65(10): 1027-1032, 2010. ilus
Article
in English
| LILACS
| ID: lil-565989
ABSTRACT
PURPOSE:
To search for anti-retina antibodies that serve as markers for eye disease in uveitis. MATERIALS ANDMETHODS:
Stored sera from patients with uveitis, ocular toxoplasmosis (n = 30) and non-infectious, immune-mediated uveitis (n = 50) and from asymptomatic individuals who were positive (n = 250) and negative (n = 250) for anti-Toxoplasma antibodies were tested. Serum anti-retina IgG was detected by an optimized ELISA using a solid-phase whole human retina extract, bovine S-antigen or interphotoreceptor retinoid-binding protein.RESULTS:
Uveitis patients showed a higher mean reactivity to whole human retina extract, interphotoreceptor retinoid-binding protein and S-antigen in comparison to the asymptomatic population. These findings were independent of the uveitis origin and allowed the determination of the lower anti-retina antibody cut-off for the three antigens. Asymptomatic anti-Toxoplasma serum-positive individuals showed a higher frequency of antihuman whole retina extract antibodies in comparison to asymptomatic anti-Toxoplasma serum-negative patients. The bovine S-antigen and interphotoreceptor retinoid-binding protein ELISAs also showed a higher mean reactivity in the uveitis groups compared to the asymptomatic group, but the observed reactivities were lower and overlapped without discrimination.CONCLUSION:
We detected higher levels of anti-retina antibodies in uveitis patients and in a small fraction of asymptomatic patients with chronic toxoplasmosis. The presence of anti-retina antibodies in sera might be a marker of eye disease in asymptomatic patients, especially when whole human retina extract is used in a solid-phase ELISA.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Retina
/
Autoantibodies
/
Uveitis
/
Toxoplasmosis
/
Toxoplasmosis, Ocular
/
Asymptomatic Infections
Type of study:
Etiology study
Limits:
Humans
Language:
English
Journal:
Clinics
Journal subject:
Medicine
Year:
2010
Type:
Article
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