Clostridium perfringens isolate typing by multiplex PCR
J. venom. anim. toxins incl. trop. dis
;
16(4): 573-578, 2010. ilus, tab
Article
in English
| LILACS, VETINDEX
| ID: lil-566155
ABSTRACT
Clostridium perfringens is an important pathogen that provokes numerous different diseases. This bacterium is classified into five different types, each of which capable of causing a different disease. There are various methods for the bacterial identification, many are labor-intensive, time-consuming, expensive and also present low sensitivity and specificity. The aim of this research was to identify the different types of C. perfringens using PCR molecular method. In this study, 130 sheep-dung samples were randomly collected from areas around the city of Kerman, southeastern Iran. After processing and culturing of samples, the produced colonies were morphologically studied, gram stain test was also carried out and the genera of these bacteria were identified through biochemical tests. DNA extracted from isolated bacteria for genotyping was tested by multiplex PCR with specific primers. Based on length of synthesized fragments by PCR, toxin types and bacterial strains were detected. C. perfringens isolated types were divided as follows 17.39 percent type A, 21.74 percent type B, 34.78 percent type C and 26.09 percent type D. It should be emphasized that, up to the present moment, C. perfringens type A has not been reported in Iran.(AU)
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Sheep
/
Biomarkers
/
Clostridium perfringens
/
Multiplex Polymerase Chain Reaction
Limits:
Animals
Language:
English
Journal:
J. venom. anim. toxins incl. trop. dis
Year:
2010
Type:
Article
Institution/Affiliation country:
Razi Vaccine and Serum Research Institute/IR
/
Shahid Bahonar University of Kerman/IR
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