Proteomic characterization of the thermostable toxins from Naja naja venom
J. venom. anim. toxins incl. trop. dis
;
16(4): 631-638, 2010. graf, tab
Article
in English
| LILACS, VETINDEX
| ID: lil-566163
ABSTRACT
Naja naja snake venom presents abundant thermostable peptides. Many of them possess useful pharmacological activity that may be employed for drug development. For the proteomic characterization of such toxins, in the present study, Naja naja venom solution was heated up to 100°C for 10, 30, 60, 120, 180 and 300 minutes and protein fractions of non-heated and heated venom were analyzed by two-dimensional nano-liquid chromatography coupled online with tandem mass spectrometry. After heating for 300 minutes, a total of 32 peptides were still detected in the supernatant. The identified peptides belong to the following groups cardiotoxins, neurotoxins and cytotoxins. It was found that thermostable peptides are able to preserve their analgesic activity after a long heating time in formalin test. Mice injected with 15 µg/g of 60-minute heated venom or with 25 µg/g of 300-minute heated venom revealed even a better analgesic activity than those treated with lidocaine.(AU)
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Peptides
/
Snake Venoms
/
Cytotoxins
/
Proteomics
/
Naja naja
Limits:
Animals
Language:
English
Journal:
J. venom. anim. toxins incl. trop. dis
Year:
2010
Type:
Article
Institution/Affiliation country:
Vietnam Academy of Science and Technology/VN
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