Cultivo y Caracterización In Vitro de una Línea Celular de Fibroblastos Gingivales Humanos / In vitro culture and characterization of a cell line of human gingival fibroblasts
Rev. Estomat
;
9(1): 27-32, jul. 2000. ilus
Article
in Spanish
| LILACS
| ID: lil-569553
ABSTRACT
The main goal is to establish a cellular line of fibroblast which come from human gingival tissue, identifying its morphologic featores. Samples were taken from gum of healthy patients without medical antecedents. Three females and three male were selected. Ages were between 16 and 30 years old. A sample of 0,5 cm² of gum was obtained to take it to a cellular growth environment. Cultures were incobated at 37C, CO² atmosphere at 5% for ten (10) days. Samples were observed daily with inverted light Microscope Olimpus II. At 6th day growth of fusiform cells was observed; their morphology was in coincidence with that of the fibroblasts, At 18th day a monolayer of 100% purity was observed. The cellular line was preserved and stored by freezing in liquid nitrogen.
Full text:
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Index:
LILACS (Americas)
Main subject:
Cell Line
/
Fibroblasts
Language:
Spanish
Journal:
Rev. Estomat
Journal subject:
Dentistry
Year:
2000
Type:
Article
Affiliation country:
Colombia
Institution/Affiliation country:
Universidad del Valle/CO
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