Effects of low-level laser therapy on human osteoblastic cells grown on titanium
Braz. dent. j
;
21(6): 491-498, 2010. ilus, tab
Article
in English
| LILACS
| ID: lil-572294
ABSTRACT
The aim of this study was to investigate the effects of low-level laser therapy (LLLT) by using gallium aluminum arsenide (GaAlAs) diode laser on human osteoblastic cells grown on titanium (Ti). Osteoblastic cells were obtained by enzymatic digestion of human alveolar bone and cultured on Ti discs for up to 17 days. Cells were exposed to LLLT at 3 J/cm2 (wavelength of 780 nm) at days 3 and 7 and non-irradiated cultures were used as control. LLLT treatment did not influence culture growth, ALP activity, and mineralized matrix formation. Analysis of cultures by epifluorescence microscopy revealed an area without cells in LLLT treated cultures, which was repopulated latter with proliferative and less differentiated cells. Gene expression of ALP, OC, BSP, and BMP-7 was higher in LLLT treated cultures, while Runx2, OPN, and OPG were lower. These results indicate that LLLT modulates cell responses in a complex way stimulating osteoblastic differentiation, which suggests possible benefits on implant osseointegration despite a transient deleterious effect immediately after laser irradiation.
Full text:
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Index:
LILACS (Americas)
Main subject:
Osteoblasts
/
Bone Matrix
/
Gene Expression
/
Osseointegration
/
Low-Level Light Therapy
Limits:
Humans
Language:
English
Journal:
Braz. dent. j
Journal subject:
Dentistry
Year:
2010
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
University of São Paulo/BR
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