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Effects of low-level laser therapy on human osteoblastic cells grown on titanium
Petri, Alice Dias; Teixeira, Lucas Novaes; Crippa, Grasiele Edilaine; Beloti, Marcio Mateus; Oliveira, Paulo Tambasco de; Rosa, Adalberto Luiz.
  • Petri, Alice Dias; University of São Paulo. Ribeirão Preto Dental School. Cell Culture Laboratory. Ribeirão Preto. BR
  • Teixeira, Lucas Novaes; University of São Paulo. Ribeirão Preto Dental School. Cell Culture Laboratory. Ribeirão Preto. BR
  • Crippa, Grasiele Edilaine; University of São Paulo. Ribeirão Preto Dental School. Cell Culture Laboratory. Ribeirão Preto. BR
  • Beloti, Marcio Mateus; University of São Paulo. Ribeirão Preto Dental School. Cell Culture Laboratory. Ribeirão Preto. BR
  • Oliveira, Paulo Tambasco de; University of São Paulo. Ribeirão Preto Dental School. Cell Culture Laboratory. Ribeirão Preto. BR
  • Rosa, Adalberto Luiz; University of São Paulo. Ribeirão Preto Dental School. Cell Culture Laboratory. Ribeirão Preto. BR
Braz. dent. j ; 21(6): 491-498, 2010. ilus, tab
Article in English | LILACS | ID: lil-572294
ABSTRACT
The aim of this study was to investigate the effects of low-level laser therapy (LLLT) by using gallium aluminum arsenide (GaAlAs) diode laser on human osteoblastic cells grown on titanium (Ti). Osteoblastic cells were obtained by enzymatic digestion of human alveolar bone and cultured on Ti discs for up to 17 days. Cells were exposed to LLLT at 3 J/cm2 (wavelength of 780 nm) at days 3 and 7 and non-irradiated cultures were used as control. LLLT treatment did not influence culture growth, ALP activity, and mineralized matrix formation. Analysis of cultures by epifluorescence microscopy revealed an area without cells in LLLT treated cultures, which was repopulated latter with proliferative and less differentiated cells. Gene expression of ALP, OC, BSP, and BMP-7 was higher in LLLT treated cultures, while Runx2, OPN, and OPG were lower. These results indicate that LLLT modulates cell responses in a complex way stimulating osteoblastic differentiation, which suggests possible benefits on implant osseointegration despite a transient deleterious effect immediately after laser irradiation.
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Full text: Available Index: LILACS (Americas) Main subject: Osteoblasts / Bone Matrix / Gene Expression / Osseointegration / Low-Level Light Therapy Limits: Humans Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2010 Type: Article Affiliation country: Brazil Institution/Affiliation country: University of São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Osteoblasts / Bone Matrix / Gene Expression / Osseointegration / Low-Level Light Therapy Limits: Humans Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2010 Type: Article Affiliation country: Brazil Institution/Affiliation country: University of São Paulo/BR