Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell lines
Genet. mol. biol
;
34(1): 6-10, 2011. ilus, tab
Article
in English
| LILACS
| ID: lil-573711
ABSTRACT
Thyroid transcription factor-1 (TTF-1, NKX2-1) is a homeodomain-containing transcriptional factor that binds to and activates the promoters of thyroid and lung-specific genes, such as thyroglobulin, thyroid peroxidase, and thyroid stimulating hormone receptor. TTF-1 is known to play a key role in the development of the thyroid. However, the precise mechanism of TTF-1 gene transcription in human thyroid cells has not been studied. The expression of transcriptional activity in various lengths of the 5'-flanking region of the human TTF -1 gene was studied in TTF-1 positive and negative human thyroid cell lines. Increased transcriptional activity was observed in thyroid cell lines containing plasmids that coded for a sequence proximal to the transcription start site of exon 1 of the TTF-1 gene. However, we did not observe any difference in promoter activity in the region up to -2.6 kb from the proximal transcription start site of the TTF-1 gene between TTF-1 positive and negative cells. These results suggest that the proximal 5'-flanking region of the human TTF -1 gene does not contain sufficient cis-active regulatory information to direct gene expression in thyroid cells,and that other cis-or trans-acting factors participate in the thyroid specific gene expression of TTF-1.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Thenoyltrifluoroacetone
/
Thyroid Gland
/
STAT3 Transcription Factor
/
Human Embryonic Stem Cells
Limits:
Humans
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
2011
Type:
Article
/
Project document
Affiliation country:
Japan
Institution/Affiliation country:
Kuma Hospital/JP
/
Osaka University/JP
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