Your browser doesn't support javascript.
loading
The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats
Gouveia Júnior, Amauri; Antunes, Gabriela; Maximino, Caio; Morato, Silvio.
  • Gouveia Júnior, Amauri; Universidade Federal do Pará. BR
  • Antunes, Gabriela; Okinawa Institute of Science and Technology. JP
  • Maximino, Caio; Universidade Federal do Pará. BR
  • Morato, Silvio; Universidade de São Paulo. BR
Psychol. neurosci. (Impr.) ; 2(2): 227-233, Dec. 2009. graf
Article in English | LILACS | ID: lil-574102
ABSTRACT
In order to determine the modulation of anxiolytic and panicolytic-like effects of diazepam by the hormonal cycle of female rats, male and female rats - the latter divided per estrous cycle phase (estrus, diestrus, metaestrus and proestrus) - were tested in the elevated T-maze, a behavioral model of panic and anxiety. Diazepam (0.5, 1.0 and 2.0 mg/kg) or saline solution was injected in individual animals that were submitted to one session in the elevated T-maze 25 min after drug/saline administration. The test consisted of three avoidance trials and one escape trial, separated by a 30 s interval, during which the animals were isolated in individual cages. The avoidance trials began with the animal being placed at the end of the maze's enclosed arm. The time necessary for the animal to leave the central square was considered as the response's latency. The trials that exceeded 300 s were considered as failures. Results demonstrate a decrease in the effects of diazepam in inhibitory avoidance (anxiety) trials in females in diestrus and proestrus, but no relation of gender or estrous cycle on diazepam effects on escape trials (fear). The results support the hypothesis that down-regulation of GABA A receptors by activation of nuclear estrogen receptors and induction of PKC-mediated GABA A receptor phosphorylation by activation of surface estrogen receptors in raphe neurons underlie the modulation of diazepam sensitivity by estrogen.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Anxiety / Estrous Cycle / Diazepam Limits: Animals Language: English Journal: Psychol. neurosci. (Impr.) Journal subject: Neurology / Psychology Year: 2009 Type: Article Affiliation country: Brazil / Japan Institution/Affiliation country: Okinawa Institute of Science and Technology/JP / Universidade Federal do Pará/BR / Universidade de São Paulo/BR

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: LILACS (Americas) Main subject: Anxiety / Estrous Cycle / Diazepam Limits: Animals Language: English Journal: Psychol. neurosci. (Impr.) Journal subject: Neurology / Psychology Year: 2009 Type: Article Affiliation country: Brazil / Japan Institution/Affiliation country: Okinawa Institute of Science and Technology/JP / Universidade Federal do Pará/BR / Universidade de São Paulo/BR