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Human papillomavirus false positive cytological diagnosis in low grade squamous intraepithelial lesion
Núñez-Troconis, José; Delgado, Mariela; González, Julia; Velásquez, Jesvy; Mindiola, Raimy; Whitby, Denise; Conde, Betty; Munroe, David J.
  • Núñez-Troconis, José; Hospital Manuel Noriega Trigo. Departamento de Obstetricia y Ginecología. Maracaibo. VE
  • Delgado, Mariela; Policlínica Maracaibo. Laboratorio de Patología. Maracaibo. VE
  • González, Julia; Policlínica Maracaibo. Laboratorio de Patología. Maracaibo. VE
  • Velásquez, Jesvy; Universidad del Zulia. Facultad de Medicina. Laboratorio Regional de Referencia Virológica. Maracaibo. VE
  • Mindiola, Raimy; Universidad del Zulia. Facultad de Medicina. Laboratorio Regional de Referencia Virológica. Maracaibo. VE
  • Whitby, Denise; National Cancer Institute at Frederick. Viral Oncology Section (VOS) Core Laboratory. Frederick. US
  • Conde, Betty; National Cancer Institute at Frederick. Viral Oncology Section (VOS) Core Laboratory. Frederick. US
  • Munroe, David J; National Cancer Institute at Frederick. Laboratory of Molecular Technology. Frederick. US
Invest. clín ; 50(4): 447-454, dic. 2009. tab
Article in English | LILACS | ID: lil-574442
ABSTRACT
The purpose of this study was to investigate the number of Human Papillomavirus false positive cytological diagnosis in low grade squamous intraepithelial lesions (LSIL). Three hundred and two women who assisted to an Out-Patient Gynecologic Clinic in Maracaibo, Venezuela, were recruited for this study. Each patient had the Pap smear and a cervical swab for Hybrid Capture 2 (HC2). Three cytotechnologists reviewed the Pap smears and two pathologists rescreened all of them. The cytotechnologists reported 161 (53.3 percent) Pap smears negatives for intraepithelial lesion (IL) or malignancy, and 141 cases (46.7 percent) with epithelial abnormalities. They reported 46 percent of 302 patients with HPV infection in Pap smear slides. The pathologists found that 241 (79.8 percent) Pap smears were negatives for IL or malignancy and 61 (20.2 percent), with abnormal Pap smears. They found 14.6 percent HPV infection in all Pap smears (p<0.0001; 46 percent vs 14.6 percent). The HC2 study showed that 47 samples (15.6 percent) were positive for HPV. The study found that 114 Pap smears (False Positive 85 percent) of 134 reported by the cytotechnologists and 24 (False Positive 43 percent) of 56 cytologies reported by the pathologists as LSIL, were negative for HPV infection determined by HC2 (p<0.00003). The present study suggests that the cytotechnologists overdiagnosed cellular changes associated with HPV infection in the Pap smear, increasing the FP cytological diagnosis of LSIL.
RESUMEN
El presente trabajo tuvo por objeto el investigar el número de falsos positivos reportados en la citología cervicovaginal (CCV) de la presencia del Virus del Papiloma Humano (VPH) con diagnóstico de Lesión Intraepitelial Escamosa de bajo grado (LIE-BG). Se estudiaron 302 mujeres que asistieron a la Consulta de Patología de Cuello Uterino del Hospital Manuel Noriega Trigo, en Maracaibo, Venezuela. A cada paciente se le practicaron una CCV y muestra para la captura de híbridos 2 (CH2). Tres citotecnólogos y 2 patólogos estudiaron las CCV. Los citotecnólogos reportaron 161(53,3 por ciento) de CCV negativas para lesión intraepitelial o malignidad y 141 casos (46,7 por ciento) con anomalías epiteliales. Éstos encontraron 46 por ciento de presencia de VPH en las 302 CCV. Los patólogos reportaron 241 CCV (79,8 por ciento) negativas y 61 CCV (20,2 por ciento) anormales. Estos encontraron en 14,6 por ciento de las CCV, la presencia de VPH (p < 0, 0001; 46 por ciento vs 14,6 por ciento). La CH2 mostró que 47 muestras (15, 6 por ciento) fueron positivas a VPH. Esta investigación mostró que 112 CCV de 134 (Falso Positivo 85 por ciento) reportados por los citotecnólogos y 24 de 56 CCV (Falso Positivo 43 por ciento) reportados por los patólogos como LIE-BG, fueron negativos a la infección del VPH determinados por la CH2 (p < 0,00003). La investigación sugiere un sobrediagnóstico de la presencia de cambios celulares debidos al VPH en la CCV, por parte de los citotecnólogos, incrementando los falsos positivos de la presencia del VPH en CCV con diagnóstico de LIE-BG.
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Full text: Available Index: LILACS (Americas) Main subject: Carcinoma in Situ / Uterine Cervical Dysplasia / Uterine Cervical Neoplasms / Cytological Techniques / Papillomavirus Infections / False Positive Reactions Type of study: Diagnostic study / Evaluation studies Limits: Female / Humans Language: English Journal: Invest. clín Journal subject: Biologia / Medicine / Relatos de Casos Year: 2009 Type: Article Affiliation country: United States / Venezuela Institution/Affiliation country: Hospital Manuel Noriega Trigo/VE / National Cancer Institute at Frederick/US / Policlínica Maracaibo/VE / Universidad del Zulia/VE

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Full text: Available Index: LILACS (Americas) Main subject: Carcinoma in Situ / Uterine Cervical Dysplasia / Uterine Cervical Neoplasms / Cytological Techniques / Papillomavirus Infections / False Positive Reactions Type of study: Diagnostic study / Evaluation studies Limits: Female / Humans Language: English Journal: Invest. clín Journal subject: Biologia / Medicine / Relatos de Casos Year: 2009 Type: Article Affiliation country: United States / Venezuela Institution/Affiliation country: Hospital Manuel Noriega Trigo/VE / National Cancer Institute at Frederick/US / Policlínica Maracaibo/VE / Universidad del Zulia/VE