Agrobacterium tumefaciens-transient genetic transformation of Habanero pepper (Capsicum chinense Jacq. ) leaf explants
Electron. j. biotechnol
;
13(4): 7-8, July 2010. ilus, tab
Article
in English
| LILACS
| ID: lil-577112
ABSTRACT
Most of the pepper species of the genus Capsicum have been recalcitrant to efficient Agrobacterium tumefaciens-mediated stable or transient, genetic transformation. In the present work, we optimized a protocol for transient transformation of the Habanero pepper (Capsicum chinense Jacq.) through the standardization of several experimental factors. These included the age of the plants, the temperature, the length of co-cultivation, the application of a negative (vacuum) and/or a positive (infiltration) pressure, along with micro injection, the use of acetosyringone during the bacterial culturing, and modification of the pH during the GUS assay to eliminate the endogenous beta-glucuronidase activity. The standardized protocol, which yielded nearly 55 percent fully transformed leaf explants, was used to successfully mobilize two empty binary vectors (pCAMBIA2301 and pCAMex), as well as the C. chinense cDNAs encoding the pathogenesis-related protein 10 and esterase, respectively.
Full text:
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Index:
LILACS (Americas)
Main subject:
Transformation, Genetic
/
Capsicum
/
Agrobacterium tumefaciens
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2010
Type:
Article
/
Project document
Affiliation country:
Mexico
/
United States
Institution/Affiliation country:
Centro de Investigaciones Biológicas del Noroeste/MX
/
Centro de Investigación Científica de Yucatán/MX
/
Instituto Tecnológico Superior de Calkiní/MX
/
State University of New Jersey/US
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