Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates
Braz. j. infect. dis
;
14(6): 564-568, Nov.-Dec. 2010. ilus, graf, tab
Article
in English
| LILACS
| ID: lil-578431
ABSTRACT
INTRODUCTION:
Resistance to macrolides, lincosamides and streptogramins B (MLS B antibiotics) in staphylococci may be due to modification in ribosomal target methylase encoded by erm genes. The expression of MLS B resistance lead to three phenotypes, namely constitutive resistance (cMLS B), inducible resistance (iMLS B), and resistance only to macrolides and streptogramins B (MS B). The iMLS B resistance is the most difficult to detect in the clinical laboratory.OBJECTIVE:
This study investigated the expression of MLS B resistance and the prevalence of the erm genes among 152 clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus (CNS) from Hospital de Clínicas de Porto Alegre.METHODS:
Primary MLS B resistance was detected by the disk diffusion method. Isolates with iMLS B phenotype were tested by double-disk induction method. All isolates were tested by a genotypic assay, PCR with specific primers.RESULTS:
A total of 46.7 percent of staphylococci were positive for cMLS B; 3.3 percent for iMLS B and 3.3 percent for MS B. One or more erm genes were present in 50.1 percent of isolates. The gene ermA was detected in 49 isolates, ermC in 29 and ermB in 3.CONCLUSION:
The prevalence of the ermA, ermB and ermC genes were 29.6 percent, 17.1 percent and 0.66 percent respectively, and constitutive resistance was the most frequent as compared to the other two phenotypes.
Full text:
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Index:
LILACS (Americas)
Main subject:
Staphylococcus
/
Macrolides
/
Drug Resistance, Bacterial
/
Genes, Bacterial
/
Anti-Bacterial Agents
Type of study:
Prevalence study
/
Risk factors
Language:
English
Journal:
Braz. j. infect. dis
Journal subject:
Communicable Diseases
Year:
2010
Type:
Article
Affiliation country:
Brazil
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