Periodontal ligament and gingival fibroblasts participate in the production of TGF-β, interleukin (IL)-8 and IL-10
Braz. oral res
;
25(2): 157-162, Mar.-Apr. 2011. ilus
Article
in English
| LILACS
| ID: lil-583850
ABSTRACT
The aim of this study was to quantify and compare the production of transforming growth factor beta (TGF-β), interleukin (IL)-8 and IL-10 by human cultured periodontal ligament and gingival fibroblasts both obtained from the same donors challenged with lipopolysaccharide (LPS) from Porphyromonas gingivalis. Fibroblasts were exposed to 0.1-10 µg/mL of LPS from P. gingivalis and after 24 h the supernatants were collected and analyzed by enzyme-linked immunosorbent assay (ELISA). TGF-β protein production was upregulated in a concentration-dependent manner, mainly in gingival fibroblasts, which was statistically significant when challenged by 10 µg/mL LPS. Additionally, at this concentration, gingival fibroblasts had almost a two-fold increase in the amount of TGF-β when compared to periodontal ligament fibroblasts. Both periodontal ligament and gingival fibroblasts showed an increase in IL-8 production when challenged with 1 µg/mL and 10 µg/mL LPS. IL-10 production remained unaffected when challenged by any of the LPS concentrations tested in either periodontal ligament or gingival fibroblasts. Our results demonstrate that periodontal ligament and gingival fibroblasts when challenged by LPS from P. gingivalis with 24 h may play a critical role in producing TGF-β and IL-8 but not IL-10.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Periodontal Ligament
/
Transforming Growth Factor beta
/
Fibroblasts
/
Gingiva
Limits:
Humans
Language:
English
Journal:
Braz. oral res
Journal subject:
Dentistry
Year:
2011
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of Rio de Janeiro/BR
/
University of São Paulo/BR
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