Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
Mem. Inst. Oswaldo Cruz
;
106(2): 139-145, Mar. 2011. ilus, tab
Article
in English
| LILACS
| ID: lil-583936
ABSTRACT
We used a colorimetric reverse dot blot hybridization (CRDH) assay to detect the presence of mutations in a specific region of the rpoB gene, associated with rifampin (RIF) resistance, in a panel of 156 DNAs extracted from 103 RIF-sensitive and 53 RIF-resistant cultures of Mycobacterium tuberculosis. When compared with the antimicrobial susceptibility test (AST), the sensitivity and specificity of the CRDH were 92.3 percent and 98.1 percent, respectively. When compared with sequencing, the sensitivity and specificity of the CRDH were 90.6 percent and 100 percent, respectively. To evaluate the performance of the assay directly in clinical specimens, 30 samples from tuberculosis patients were used. For these samples, the results of the CRDH were 100 percent consistent with the results of the AST and sequencing. These results indicate that the rate of concordance of the CRDH is high when compared to conventional methods and sequencing data. The CRDH can be successfully applied when a rapid test is required for the identification of RIF resistance in M. tuberculosis.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Rifampin
/
Bacterial Proteins
/
DNA, Bacterial
/
Drug Resistance, Bacterial
/
Antibiotics, Antitubercular
/
Mutation
/
Mycobacterium tuberculosis
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2011
Type:
Article
Affiliation country:
Brazil
/
Italy
Institution/Affiliation country:
Centro Regionale di Riferimento per i Micobatteri/IT
/
Fundação Estadual de Produção e Pesquisa em Saúde/BR
/
Instituto Oswaldo Cruz-Fiocruz/BR
/
Universidade Federal do Rio Grande do Sul/BR
/
Universidade de Caxias do Sul/BR
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