An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens
Mem. Inst. Oswaldo Cruz
;
106(2): 182-189, Mar. 2011. graf, tab
Article
in English
| LILACS
| ID: lil-583943
ABSTRACT
Domestic dogs are considered to be the main reservoirs of zoonotic visceral leishmaniasis. In this work, we evaluated a protocol to induce Leishmania infantum/Leishmania chagasi-specific cellular and humoral immune responses in dogs, which consisted of two injections of Leishmania promastigote lysate followed by a subcutaneous inoculation of viable promastigotes. The primary objective was to establish a canine experimental model to provide positive controls for testing immune responses to Leishmania in laboratory conditions. After inoculation of viable promastigotes, specific proliferative responses of peripheral blood mononuclear cells (PBMCs) to either Leishmania lysate or recombinant proteins, the in vitro production of interferon-γ by antigen-stimulated PBMCs and a significant increase in circulating levels of anti-Leishmania antibodies were observed. The immunized dogs also displayed positive delayed-type hypersensitivity reactions to Leishmania crude antigens and to purified recombinant proteins. An important finding that supports the suitability of the dogs as positive controls is that they remained healthy for the entire observation period, i.e., more than seven years after infection. Following the Leishmania antigen lysate injections, the infection of dogs by the subcutaneous route appears to induce a sustained cellular immune response, leading to an asymptomatic infection. This provides a useful model for both the selection of immunogenic Leishmania antigens and for immunobiological studies on their possible immunoprotective activities.
Full text:
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Index:
LILACS (Americas)
Main subject:
Antibodies, Protozoan
/
Leishmania infantum
/
Dog Diseases
/
Immunity, Cellular
/
Leishmaniasis, Visceral
/
Antigens, Protozoan
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2011
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Centro de Pesquisa Gonçalo Moniz-Fiocruz/BR
/
Universidade Federal da Bahia/BR
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