2D-immunoblotting analysis of Sporothrix schenckii cell wall
Mem. Inst. Oswaldo Cruz
;
106(2): 248-250, Mar. 2011. ilus
Article
in English
| LILACS
| ID: lil-583953
ABSTRACT
We utilized two-dimensional gel electrophoresis and immunoblotting (2D-immunoblotting) with anti-Sporothrix schenckii antibodies to identify antigenic proteins in cell wall preparations obtained from the mycelial and yeast-like morphologies of the fungus. Results showed that a 70-kDa glycoprotein (Gp70) was the major antigen detected in the cell wall of both morphologies and that a 60-kDa glycoprotein was present only in yeast-like cells. In addition to the Gp70, the wall from filament cells showed four proteins with molecular weights of 48, 55, 66 and 67 kDa, some of which exhibited several isoforms. To our knowledge, this is the first 2D-immunoblotting analysis of the S. schenckii cell wall.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Sporothrix
/
Membrane Glycoproteins
/
Cell Wall
/
Antigens, Fungal
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2011
Type:
Article
Affiliation country:
Mexico
Institution/Affiliation country:
Universidad Juárez del Estado de Durango/MX
/
Universidad Nacional Autónoma de México/MX
/
Universidad de Guanajuato/MX
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