In vitro evaluation of the action of irrigating solutions associated with intracanal medications on Escherichia coli and its endotoxin in root canals
J. appl. oral sci
;
19(2): 106-112, May-Apr. 2011. tab
Article
in English
| LILACS
| ID: lil-586030
ABSTRACT
OBJECTIVE:
The purpose of this study was to evaluate the effcacy of auxiliary chemical substances and intracanal medications on Escherichia coli and its endotoxin in root canals. MATERIAL ANDMETHODS:
Teeth were contaminated with a suspension of E. coli for 14 days and divided into 3 groups according to the auxiliary chemical substance used G1) 2.5 percent sodium hypochlorite (NaOCl); G2) 2 percent chlorhexidine gel (CLX); G3) pyrogenfree solution. After, these groups were subdivided according to the intracanal medication (ICM) A) Calcium hydroxide paste (Calen®), B) polymyxin B, and C) Calcium hydroxide paste+2 percent CLX gel. For the control group (G4), pyrogen-free saline solution was used without application of intracanal medication. Samples of the root canal content were collected immediately after biomechanical preparation (BMP), at 7 days after BMP, after 14 days of intracanal medication activity, and 7 days after removal of intracanal medication. The following aspects were evaluated for all collections a) antimicrobial activity; b) quantifcation of endotoxin by the Limulus Amebocyte Lysate test (LAL). Results were analyzed by the Kruskal-Wallis and Dunn's tests at 5 percent signifcance level.RESULTS:
The 2.5 percent NaOCl and CLX were able to eliminate E. coli from root canal lumen and reduced the amount of endotoxin compared to saline.CONCLUSIONS:
It was concluded that 2.5 percent NaOCl and CLX were effective in eliminating E. coli. Only the studied intracanal medications were to reduce the amount of endotoxin present in the root canals, regardless of the irrigant used.
Full text:
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Index:
LILACS (Americas)
Main subject:
Root Canal Irrigants
/
In Vitro Techniques
/
Dental Pulp Cavity
/
Endotoxins
/
Escherichia coli
Type of study:
Risk factors
Limits:
Humans
Language:
English
Journal:
J. appl. oral sci
Journal subject:
Dentistry
Year:
2011
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
São Paulo State University/BR
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