Effect of nitric oxide inhibitor and donor substances on the infammatory process caused by endodontic irrigants
J. appl. oral sci
;
19(2): 113-117, May-Apr. 2011. ilus, tab
Article
in English
| LILACS
| ID: lil-586042
ABSTRACT
Nitric oxide (NO) has been considered a key molecule in infammation. OBJECTIVE:
The aim of this study was to evaluate the effect of treatment with L-NAME and sodium nitroprussiate, substances that inhibit and release NO, respectively, on tissue tolerance to endodontic irrigants. MATERIAL ANDMETHODS:
The vital dye exudation method was used in a rat subcutaneous tissue model. Injections of 2 percent Evans blue were administered intravenously into the dorsal penial vein of 14 male rats (200-300 g). The NO inhibitor and donor substances were injected into the subcutaneous tissue in the dorsal region, forming two groups of animals G1 was inoculated with L-NAME and G2 with sodium nitroprussiate. Both groups received injections of the test endodontic irrigants acetic acid, 15 percent citric acid, 17 percent EDTA-T and saline (control). After 30 min, analysis of the extravasated dye was performed by light absorption spectrophotometry (620 nm).RESULTS:
There was statistically signifcant difference (p<0.05) between groups 1 and 2 for all irrigants. L-NAME produced a less intense infammatory reaction and nitroprussiate intensifed this process.CONCLUSIONS:
Independently of the administration of NO inhibitors and donors, EDTA-T produced the highest irritating potential in vital tissue among the tested irrigating solutions.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Root Canal Irrigants
/
Nitroprusside
/
NG-Nitroarginine Methyl Ester
/
Nitric Oxide Donors
/
Enzyme Inhibitors
/
Nitric Oxide
Limits:
Animals
Language:
English
Journal:
J. appl. oral sci
Journal subject:
Dentistry
Year:
2011
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
University of São Paulo/BR
/
University of Taubaté/BR
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