Reduction of the amount of intestinal secretory IgA in fulminant hepatic failure
Braz. j. med. biol. res
;
44(5): 477-482, May 2011. ilus
Article
in English
| LILACS
| ID: lil-586510
ABSTRACT
Intestinal barrier dysfunction plays an important role in spontaneous bacterial peritonitis. In the present study, changes in the intestinal barrier with regard to levels of secretory immunoglobulin A (SIgA) and its components were studied in fulminant hepatic failure (FHF). Immunohistochemistry and double immunofluorescent staining were used to detect intestinal IgA, the secretory component (SC) and SIgA in patients with FHF (20 patients) and in an animal model with FHF (120 mice). Real-time PCR was used to detect intestinal SC mRNA in the animal model with FHF. Intestinal SIgA, IgA, and SC staining in patients with FHF was significantly weaker than in the normal control group (30 patients). Intestinal IgA and SC staining was significantly weaker in the animal model with FHF than in the control groups (normal saline 30 mice; lipopolysaccharide 50 mice; D-galactosamine 50 mice; FHF 120 mice). SC mRNA of the animal model with FHF at 2, 6, and 9 h after injection was 0.4 ± 0.02, 0.3 ± 0.01, 0.09 ± 0.01, respectively. SC mRNA of the animal model with FHF was significantly decreased compared to the normal saline group (1.0 ± 0.02) and lipopolysaccharide group (0.89 ± 0.01). The decrease in intestinal SIgA and SC induced failure of the intestinal immunologic barrier and the attenuation of gut immunity in the presence of FHF.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Immunoglobulin A, Secretory
/
Liver Failure, Acute
Type of study:
Observational study
Limits:
Animals
/
Humans
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2011
Type:
Article
Affiliation country:
Cambodia
/
China
Institution/Affiliation country:
China Medical University Affiliated Shengjing Hospital/CN
/
Ministry of Health/CN
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