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Effect of blocking Rac1 expression in cholangiocarcinoma QBC939 cells
Xudong, Liu; Guangyi, Wang.
  • Xudong, Liu; Jilin University. First Clinical Hospital. Department of General Surgery. Changchun. CN
  • Guangyi, Wang; Jilin University. First Clinical Hospital. Department of General Surgery. Changchun. CN
Braz. j. med. biol. res ; 44(5): 483-488, May 2011. ilus
Article in English | LILACS | ID: lil-586515
ABSTRACT
Cholangiocarcinomas (CCs) are malignant tumors that originate from epithelial cells lining the biliary tree and gallbladder. Ras correlative C3 creotoxin substrate 1 (Rac1), a small guanosine triphosphatase, is a critical mediator of various aspects of endothelial cell functions. The objective of the present investigation was to study the effect of blocking Rac1 expression in CCs. Seventy-four extrahepatic CC (ECC) specimens and matched adjacent normal mucosa were obtained from the Department of Pathology, Inner Mongolia Medicine Hospital, between 2007 and 2009. Our results showed that the expression of Rac1 was significantly higher (53.12 percent) in tumor tissues than in normal tissues. Western blotting data indicated a significant reduction in Rac1-miRNA cell protein levels. Rac1-miRNA cell growth rate was significantly different at 24, 48, and 72 h after transfection. Flow cytometry analysis showed that Rac1-miRNA cells undergo apoptosis more effectively than control QBC939 cells. Blocking Rac1 expression by RNAi effectively inhibits the growth of CCs. miRNA silencing of the Rac1 gene suppresses proliferation and induces apoptosis of QBC939 cells. These results suggest that Rac1 may be a new gene therapy target for CC. Blocking Rac1 expression in CC cells induces apoptosis of these tumor cells and may thus represent a new therapeutic approach.
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Full text: Available Index: LILACS (Americas) Main subject: Apoptosis / Cholangiocarcinoma / Rac1 GTP-Binding Protein / Gene Silencing / RNA, Small Interfering Limits: Humans Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2011 Type: Article Affiliation country: China Institution/Affiliation country: Jilin University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Apoptosis / Cholangiocarcinoma / Rac1 GTP-Binding Protein / Gene Silencing / RNA, Small Interfering Limits: Humans Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2011 Type: Article Affiliation country: China Institution/Affiliation country: Jilin University/CN