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Effects of Bothrops moojeni venom on Leishmania amazonensis promastigote forms
Castilhos, P; Pereira, C. G; Silva, A. L. N; Napolitano, D. R; Oliveira, F; Souza, M. A.
  • Castilhos, P; Federal University of Uberlândia. Institute of Biomedical Sciences. Laboratory of Molecular Biology. Uberlândia. BR
  • Pereira, C. G; Federal University of Uberlândia. Institute of Biomedical Sciences. Laboratory of Molecular Biology. Uberlândia. BR
  • Silva, A. L. N; Federal University of Uberlândia. Institute of Biomedical Sciences. Laboratory of Molecular Biology. Uberlândia. BR
  • Napolitano, D. R; Federal University of Uberlândia. Laboratory of Biophysics. Uberlândia. BR
  • Oliveira, F; Federal University of Uberlândia. Laboratory of Biophysics. Uberlândia. BR
  • Souza, M. A; Federal University of Uberlândia. Institute of Biomedical Sciences. Laboratory of Molecular Biology. Uberlândia. BR
J. venom. anim. toxins incl. trop. dis ; 17(2): 150-158, 2011. ilus, graf
Article in English | LILACS | ID: lil-587774
ABSTRACT
In the present work, the effect of Bothrops moojeni venom fractions on Leishmania promastigotes was evaluated. The snake venom was fractionated into five protein fractions (E1 to E5), by ion exclusion chromatography, that were used to treat Leishmania amazonensis and Leishmania braziliensis promastigote forms whereas the viability and nitric oxide production were evaluated. It was observed that E5 venom fraction strongly inhibited Leishmania amazonensis nitric oxide production, while in Leishmania braziliensis the nitric oxide production was enhanced in all doses. Bothrops moojeni crude venom reduced the viability of both parasites in a dose-dependent manner and a peptide of 64 kDa was apparently degraded. Bothrops moojeni E5 venom fraction only reduced the viability and nitric oxide production of Leishmania amazonensis and no protein degradation was observed. Thus, these results suggest that Bothrops moojeni E5 venom fraction may offer components with a promising antileishmanial therapeutic application.
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Full text: Available Index: LILACS (Americas) Main subject: Bothrops / Crotalid Venoms / Leishmania / Nitric Oxide Limits: Animals Language: English Journal: J. venom. anim. toxins incl. trop. dis Journal subject: Toxicology Year: 2011 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Uberlândia/BR

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Full text: Available Index: LILACS (Americas) Main subject: Bothrops / Crotalid Venoms / Leishmania / Nitric Oxide Limits: Animals Language: English Journal: J. venom. anim. toxins incl. trop. dis Journal subject: Toxicology Year: 2011 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Uberlândia/BR