Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil
Braz. j. infect. dis
;
15(3): 195-199, May-June 2011. tab
Article
in English
| LILACS
| ID: lil-589947
ABSTRACT
Infection by Pseudomonas aeruginosa has spread worldwide, with limited options for treatment. The purpose of this study was to investigate metallo-β-lactamase-producing P. aeruginosa strains and compare their genetic profile using samples collected from patients in intensive care units. Forty P. aeruginosa strains were isolated from two public hospitals in Campo Grande, Mato Grosso do Sul State, from January 1st, 2007 to June 31st, 2008. Profiles of antimicrobial susceptibility were determined using the agar diffusion method. Metallo-β-lactamase was investigated using the double-disk diffusion test and PCR. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE). Respiratory and urinary tracts were the most common isolation sites. Of the 40 samples tested, 72.5 percent (29/40) were resistant to ceftazidime and 92.5 percent (37/40) to imipenem, whereas 65 percent (26/40) were resistant to both antimicrobials. Fifteen pan-resistant samples were found. Five percent (2/40) of samples were positive for metallo-β-lactamase on the phenotype test. No metallo-β-lactamase subtype was detected by PCR. Macrorestriction analysis revealed 14 distinct genetic patterns. Based on the superior accuracy of PCR, it can be inferred that P. aeruginosa isolates from the investigated hospitals have alternative mechanisms of carbapenem resistance. The results also suggest clonal spread of P. aeruginosa between the studied hospitals.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Pseudomonas aeruginosa
/
Pseudomonas Infections
/
Beta-Lactamases
/
Beta-Lactam Resistance
Limits:
Adult
/
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Braz. j. infect. dis
Journal subject:
Communicable Diseases
Year:
2011
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
FIOCRUZ/BR
/
Hospital Regional de Mato Grosso do Sul/BR
/
Universidade Federal de Mato Grosso do Sul/BR
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