Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping

Miyata, Marcelo; Santos, Adolfo Carlos Barreto; Mendes, Natália Helena; Cunha, Eunice Atsuko; Melo, Fernando Augusto Fiúza de; Leite, Clarice Queico Fujimura

Miyata, Marcelo; Santos, Adolfo Carlos Barreto; Mendes, Natália Helena; Cunha, Eunice Atsuko; Melo, Fernando Augusto Fiúza de; Leite, Clarice Queico Fujimura.

Miyata, Marcelo; Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Araraquara. BR
Santos, Adolfo Carlos Barreto; Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Araraquara. BR
Mendes, Natália Helena; s.af
Cunha, Eunice Atsuko; s.af
Melo, Fernando Augusto Fiúza de; s.af
Leite, Clarice Queico Fujimura; s.af

Braz. j. microbiol ; 42(2): 774-777, Apr.-June 2011. ilus

Article in English | LILACS | ID: lil-590022

ABSTRACT

ABSTRACT

We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.

CTAB; DNA extraction; Mycobacterium tuberculosis; Thermolysis

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Full text: Available Index: LILACS (Americas) Type of study: Diagnostic study Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2011 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual Paulista/BR

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