Identification and antimicrobial resistance of microflora colonizing feral pig (Sus scrofa) of Brazilian Pantanal

ABSTRACT

Antimicrobial resistance of bacteria is a worldwide problem affecting wild life by living with resistant bacteria in the environment. This study presents a discussion of outside factors environment on microflora of feral pigs (Sus scrofa) from Brazilian Pantanal. Animals had samples collected from six different body sites coming from two separated geographic areas, Nhecolandia and Rio Negro regions. With routine biochemical tests and commercial kits 516 bacteria were identified, with 240 Gram-positive, predominantly staphylococci (36) and enterococci (186) strains. Among Gram-negative (GN) bacteria the predominant specimens of Enterobacteriaceae (247) mainly represented by Serratia spp. (105), Escherichia coli (50), and Enterobacter spp. (40) and specimens not identified (7). Antimicrobial susceptibility was tested against 17 drugs by agar diffusion method. Staphylococci were negative to production of enterotoxins and TSST-1, with all strains sensitive towards four drugs and highest resistance toward ampicillin (17 percent). Enterococci presented the highest sensitivity against vancomycin (98 percent), ampicillin (94 percent) and tetracycline (90 percent), and highest resistance pattern toward oxacillin (99 percent), clindamycin (83 percent), and cotrimoxazole (54 percent). In GN the highest resistance was observed with Serratia marcescens against CFL (98 percent), AMC (66 percent) and AMP (60 percent) and all drugs was most effective against E. coli SUT, TET (100 percent), AMP, TOB (98 percent), GEN, CLO (95 percent), CFO, CIP (93 percent). The results show a new profile of oxacillin-resistant enterococci from Brazilian feral pigs and suggest a limited residue and spreading of antimicrobials in the environment, possibly because of low anthropogenic impact reflected by the drug susceptibility profile of bacteria isolated.