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In vitro development of bovine embryos cultured under different fetal calf serum concentrations and cell types / Desenvolvimento in vitro de embriões bovinos cultivados sob diferentes concentrações de soro fetal bovino e tipos celulares
Tavares, Lilian Mara Trevisan; Feitosa, Weber Beringui; Oliveira, Viviani Purri de; Nichi, Marcilio; Assumpção, Mayra Elena Ortiz D'Ávila; Visintin, José Antonio.
  • Tavares, Lilian Mara Trevisan; University of São Paulo. College of Veterinary Medicine and Animal Science. Department of Animal Reproduction. São Paulo. BR
  • Feitosa, Weber Beringui; University of São Paulo. College of Veterinary Medicine and Animal Science. Department of Animal Reproduction. São Paulo. BR
  • Oliveira, Viviani Purri de; University of São Paulo. College of Veterinary Medicine and Animal Science. Department of Animal Reproduction. São Paulo. BR
  • Nichi, Marcilio; University of São Paulo. College of Veterinary Medicine and Animal Science. Department of Animal Reproduction. São Paulo. BR
  • Assumpção, Mayra Elena Ortiz D'Ávila; University of São Paulo. College of Veterinary Medicine and Animal Science. Department of Animal Reproduction. São Paulo. BR
  • Visintin, José Antonio; University of São Paulo. College of Veterinary Medicine and Animal Science. Department of Animal Reproduction. São Paulo. BR
Braz. j. vet. res. anim. sci ; 48(1): 38-45, 2011. tab
Article in English | LILACS | ID: lil-591492
ABSTRACT
The present work was carried out to evaluate the in vitro development of bovine embryo co-cultured in granulosa, oviduct, BRL or VERO cells co-cultures, supplemented with 5% or 10% of Fetal Calf Serum (FCS). Cummulus oocyte complexes were aspirated, matured and fertilized in vitro. Embryonic structures were divided into eight treatments. They were placed in culture media TCM 199 containing granulosa, oviduct, BRL or VERO cells, each of them added with 5% or 10% FCS. The conditions for the co-culture were 38.5 ºC, 5% CO2 in air and high humidity for ten consecutive days. Cleavage, blastocyst and hatching rates did not differ (p > 0.05) in co-culture with primary cells (granulosa and oviduct) when FCS concentration increased from 5 to 10%. However, in continuous cells co-culture (BRL and VERO), when FCS concentration increased from 5% to 10%, the blastocyst development rate decreased significantly (p < 0.05) from 33.6 to 16.3% and from 40 to 16.5% in embryo co-culture with BRL and VERO cells, respectively.
RESUMO
O presente trabalho foi realizado com o objetivo de avaliar o desenvolvimento in vitro de embriões bovinos co-cultivados em células da granulosa, do oviduto, BRL e VERO, suplementados com 5% ou 10% de Soro Fetal Bovino (SFB). Os complexos cummulus oócitos foram aspirados, maturados e fecundados in vitro. As estruturas embrionárias foram divididas em oito tratamentos co-cultivo em TCM 199 contendo células da granulosa, do oviduto, BRL ou VERO adicionadas com 5% ou 10% de SFB. As condições de cultivo foram 38.5 ºC, 5% CO2 em ar e alta humidade por dez dias consecutivos. Os índices de clivagem, blastocisto e eclosão não diferiram (p > 0,05) no co-cultivo com células primárias (granulosa e oviduto) quando a concentração de SFB aumentou de 5 para 10%. Entretanto, no co-cultivo com células de linhagens contínuas (BRL e VERO), quando a concentração de SFB aumentou de 5% para 10%, os índices de blastocistos diminuíram significativamente (p < 0,05) de 33,6 para 16,3 % e de 40 para 16,5% nos embriões bovinos co-cultivados com células VERO e BRL, respectivamente.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Blastocyst / Cattle Limits: Animals Language: English Journal: Braz. j. vet. res. anim. sci Journal subject: Veterinary Medicine Year: 2011 Type: Article Affiliation country: Brazil Institution/Affiliation country: University of São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Blastocyst / Cattle Limits: Animals Language: English Journal: Braz. j. vet. res. anim. sci Journal subject: Veterinary Medicine Year: 2011 Type: Article Affiliation country: Brazil Institution/Affiliation country: University of São Paulo/BR