Detection of single nucleotide polymorphisms in the conserved ESTs regions of Gossypium arboreum
Electron. j. biotechnol
;
13(5): 3-4, Sept. 2010. ilus, tab
Article
in English
| LILACS
| ID: lil-591885
ABSTRACT
Exploring genetic variation in Gossypium arboreum L. germplasm is useful as it contains many important genes conferring resistance to different stresses. In limited earlier studies, low level of genetic diversity was found by using conventional DNA marker systems which may impede future genome mapping studies. In the present investigation, we explored the extent of Single Nucleotide Polymorphisms (SNP) among 30 conserved regions of Expressed Sequence Tags (EST) of low copy genes between two genotypes of G. arboreum. A total of 27 SNPs including 21 substitutions and 6 Insertions and deletions (Indels) in 7804 bp were found between these genotypes with a frequency of one SNP per 371 bp and one Indel after every 1300 bp. Out of these SNPs, 52 percent were transitions, whilst 48 percent SNPs were transversion. In conclusion, SNPs are expedient markers that can explore polymorphism in highly conserved sequences where other markers are not effective.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Gossypium
/
Polymorphism, Single Nucleotide
Type of study:
Diagnostic study
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2010
Type:
Article
Affiliation country:
Pakistan
Institution/Affiliation country:
National Institute for Biotechnology and Genetic Engineering/PK
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