An MLSA-based online scheme for the rapid identification of Stenotrophomonas isolates
Mem. Inst. Oswaldo Cruz
;
106(4): 394-399, June 2011. ilus, tab
Article
in English
| LILACS
| ID: lil-592180
ABSTRACT
An online scheme to assign Stenotrophomonas isolates to genomic groups was developed using the multilocus sequence analysis (MLSA), which is based on the DNA sequencing of selected fragments of the housekeeping genes ATP synthase alpha subunit (atpA), the recombination repair protein (recA), the RNA polymerase alpha subunit (rpoA) and the excision repair beta subunit (uvrB). This MLSA-based scheme was validated using eight of the 10 Stenotrophomonas species that have been previously described. The environmental and nosocomial Stenotrophomonas strains were characterised using MLSA, 16S rRNA sequencing and DNA-DNA hybridisation (DDH) analyses. Strains of the same species were found to have greater than 95 percent concatenated sequence similarity and specific strains formed cohesive readily recognisable phylogenetic groups. Therefore, MLSA appeared to be an effective alternative methodology to amplified fragment length polymorphism fingerprint and DDH techniques. Strains of Stenotrophomonas can be readily assigned through the open database resource that was developed in the current study (www.steno.lncc.br/).
Full text:
Available
Index:
LILACS (Americas)
Main subject:
DNA, Bacterial
/
Stenotrophomonas
/
Multilocus Sequence Typing
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2011
Type:
Article
/
Project document
Affiliation country:
Belgium
/
Brazil
Institution/Affiliation country:
Ghent University/BE
/
Laboratório Nacional de Computação Científica/BR
/
Universidade Federal do Rio de Janeiro/BR
/
Universidade de São Paulo/BR
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