Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal
Mem. Inst. Oswaldo Cruz
;
106(4): 467-474, June 2011. ilus, mapas, tab
Article
in English
| LILACS
| ID: lil-592199
ABSTRACT
Despite evidence of West Nile virus (WNV) activity in Colombia, Venezuela and Argentina, this virus has not been reported in most South American countries. In February 2009, we commenced an investigation for WNV in mosquitoes, horses and caimans from the Pantanal, Central-West Brazil. The sera of 168 horses and 30 caimans were initially tested using a flaviviruses-specific epitope-blocking enzyme-linked immunosorbent assay (blocking ELISA) for the detection of flavivirus-reactive antibodies. The seropositive samples were further tested using a plaque-reduction neutralisation test (PRNT90) for WNV and its most closely-related flaviviruses that circulate in Brazil to confirm the detection of specific virus-neutralising antibodies. Of the 93 (55.4 percent) blocking ELISA-seropositive horse serum samples, five (3 percent) were seropositive for WNV, nine (5.4 percent) were seropositive for St. Louis encephalitis virus, 18 (10.7 percent) were seropositive for Ilheus virus, three (1.8 percent) were seropositive for Cacipacore virus and none were seropositive for Rocio virus using PRNT90, with a criteria of > four-fold antibody titre difference. All caimans were negative for flaviviruses-specific antibodies using the blocking ELISA. No virus genome was detected from caiman blood or mosquito samples. The present study is the first report of confirmed serological evidence of WNV activity in Brazil.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
West Nile Fever
/
West Nile virus
/
Alligators and Crocodiles
/
Antibodies, Neutralizing
/
Horse Diseases
/
Horses
/
Culicidae
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2011
Type:
Article
Affiliation country:
Argentina
/
Brazil
Institution/Affiliation country:
Instituto Nacional de Enfermidades Virales Humanas Dr Julio I Maiztegui/AR
/
Ministério da Agricultura Pecuária e Abastecimento/BR
/
Ministério da Saúde/BR
/
Universidade de São Paulo/BR
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