Histomorphometric changes in the perirenal adipocytes of adrenalectomized rats treated with dexamethasone
Clinics
;
66(5): 849-853, 2011. ilus, graf
Article
in English
| LILACS
| ID: lil-593851
ABSTRACT
INTRODUCTION:
Prolonged steroid treatment administered to any patient can cause visceral obesity, which is associated with metabolic disease and Cushing's syndrome. Glucocorticoids have a profound negative effect on adipose tissue mass, giving rise to obesity, which in turn is regulated by the 11β-hydroxysteroid dehydrogenase type 1 enzyme. Adrenalectomized rats treated with dexamethasone exhibited an increase in visceral fat deposition but not in body weight.OBJECTIVES:
The main aim of this study was to determine the effect of dexamethasone on the histomorphometric characteristics of perirenal adipocytes of adrenalectomized, dexamethasone-treated rats (ADR+Dexa) and the association of dexamethasone treatment with the expression and activity of 11 β-hydroxysteroid dehydrogenase type 1 (11 β-hydroxysteroid dehydrogenase type 1).METHODS:
A total of 20 male Sprague Dawley rats were divided into 3 groups a baseline control group (n = 6), a sham-operated group (n = 7) and an adrenalectomized group (n=7). The adrenalectomized group was given intramuscular dexamethasone (ADR+Dexa) 2 weeks post adrenalectomy, and the rats from the sham-operated group were administered intramuscular vehicle (olive oil).RESULTS:
Treatment with 120 μg/kg intramuscular dexamethasone for 8 weeks resulted in a significant decrease in the diameter of the perirenal adipocytes (p<0.05) and a significant increase in the number of perirenal adipocytes (p<0.05). There was minimal weight gain but pronounced fat deposition in the dexamethasone-treated rats. These changes in the perirenal adipocytes were associated with high expression and dehydrogenase activity of 11β-hydroxysteroid dehydrogenase type 1.CONCLUSIONS:
In conclusion, dexamethasone increased the deposition of perirenal fat by hyperplasia, which causes increases in the expression and dehydrogenase activity of 11 β-hydroxysteroid dehydrogenase type 1 in adrenalectomized rats.
Full text:
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Index:
LILACS (Americas)
Main subject:
Dexamethasone
/
Adipose Tissue
/
Adipocytes
/
Intra-Abdominal Fat
/
Glucocorticoids
/
Kidney
Limits:
Animals
Language:
English
Journal:
Clinics
Journal subject:
Medicine
Year:
2011
Type:
Article
/
Project document
Affiliation country:
Malaysia
Institution/Affiliation country:
Universiti Kebangsaan Malaysia/MY
/
Universiti Tunku Abdul Rahman/MY
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