Heparanase isoform expression and extracellular matrix remodeling in intervertebral disc degenerative disease
Clinics
;
66(5): 903-909, 2011. ilus, graf, tab
Article
in English
| LILACS
| ID: lil-593858
ABSTRACT
OBJECTIVE:
To determine the molecules involved in extracellular matrix remodeling and to identify and quantify heparanase isoforms present in herniated and degenerative discs.INTRODUCTION:
Heparanase is an endo-beta-glucuronidase that specifically acts upon the heparan sulfate chains of proteoglycans. However, heparanase expression in degenerative intervertebral discs has not yet been evaluated. Notably, previous studies demonstrated a correlation between changes in the heparan sulfate proteoglycan pattern and the degenerative process associated with intervertebral discs.METHODS:
Twenty-nine samples of intervertebral degenerative discs, 23 samples of herniated discs and 12 samples of non-degenerative discs were analyzed. The expression of both heparanase isoforms (heparanase-1 and heparanase-2) was evaluated using immunohistochemistry and real-time RT-PCR analysis.RESULTS:
Heparanase-1 and heparanase-2 expression levels were significantly higher in the herniated and degenerative discs in comparison to the control tissues, suggesting a possible role of these proteins in the intervertebral degenerative process.CONCLUSION:
The overexpression of heparanase isoforms in the degenerative intervertebral discs and the herniated discs suggests a potential role of both proteins in the mediation of inflammatory processes and in extracellular matrix remodeling. The heparanase-2 isoform may be involved in normal metabolic processes, as evidenced by its higher expression in the control intervertebral discs relative to the expression of heparanase-1.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Extracellular Matrix
/
Intervertebral Disc Degeneration
/
Glucuronidase
/
Intervertebral Disc Displacement
Type of study:
Observational study
/
Risk factors
Limits:
Adolescent
/
Adult
/
Humans
Language:
English
Journal:
Clinics
Journal subject:
Medicine
Year:
2011
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Faculdade de Medicina ABC/BR
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