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Heparanase isoform expression and extracellular matrix remodeling in intervertebral disc degenerative disease
Rodrigues, Luciano Miller Reis; Theodoro, Thérèse Rachell; Matos, Leandro Luongo; Mader, Ana Maria; Milani, Carlo; Pinhal, Maria Aparecida da Silva.
  • Rodrigues, Luciano Miller Reis; Faculdade de Medicina ABC. Orthopedic Department. Santo André. BR
  • Theodoro, Thérèse Rachell; Faculdade de Medicina ABC. Biochemistry Department. Santo André. BR
  • Matos, Leandro Luongo; Faculdade de Medicina ABC. Biochemistry Department. Santo André. BR
  • Mader, Ana Maria; Faculdade de Medicina ABC. Pathology Department. Santo André. BR
  • Milani, Carlo; Faculdade de Medicina ABC. Orthopedic Department. Santo André. BR
  • Pinhal, Maria Aparecida da Silva; Faculdade de Medicina ABC. Biochemistry Department. Santo André. BR
Clinics ; 66(5): 903-909, 2011. ilus, graf, tab
Article in English | LILACS | ID: lil-593858
ABSTRACT

OBJECTIVE:

To determine the molecules involved in extracellular matrix remodeling and to identify and quantify heparanase isoforms present in herniated and degenerative discs.

INTRODUCTION:

Heparanase is an endo-beta-glucuronidase that specifically acts upon the heparan sulfate chains of proteoglycans. However, heparanase expression in degenerative intervertebral discs has not yet been evaluated. Notably, previous studies demonstrated a correlation between changes in the heparan sulfate proteoglycan pattern and the degenerative process associated with intervertebral discs.

METHODS:

Twenty-nine samples of intervertebral degenerative discs, 23 samples of herniated discs and 12 samples of non-degenerative discs were analyzed. The expression of both heparanase isoforms (heparanase-1 and heparanase-2) was evaluated using immunohistochemistry and real-time RT-PCR analysis.

RESULTS:

Heparanase-1 and heparanase-2 expression levels were significantly higher in the herniated and degenerative discs in comparison to the control tissues, suggesting a possible role of these proteins in the intervertebral degenerative process.

CONCLUSION:

The overexpression of heparanase isoforms in the degenerative intervertebral discs and the herniated discs suggests a potential role of both proteins in the mediation of inflammatory processes and in extracellular matrix remodeling. The heparanase-2 isoform may be involved in normal metabolic processes, as evidenced by its higher expression in the control intervertebral discs relative to the expression of heparanase-1.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Extracellular Matrix / Intervertebral Disc Degeneration / Glucuronidase / Intervertebral Disc Displacement Type of study: Observational study / Risk factors Limits: Adolescent / Adult / Humans Language: English Journal: Clinics Journal subject: Medicine Year: 2011 Type: Article Affiliation country: Brazil Institution/Affiliation country: Faculdade de Medicina ABC/BR

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Full text: Available Index: LILACS (Americas) Main subject: Extracellular Matrix / Intervertebral Disc Degeneration / Glucuronidase / Intervertebral Disc Displacement Type of study: Observational study / Risk factors Limits: Adolescent / Adult / Humans Language: English Journal: Clinics Journal subject: Medicine Year: 2011 Type: Article Affiliation country: Brazil Institution/Affiliation country: Faculdade de Medicina ABC/BR