Influence of 2-methoxyestradiol on MCF-7 cells: an improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels
Biocell
;
33(1): 67-70, Apr. 2009. ilus, graf
Article
in English
| LILACS
| ID: lil-595023
ABSTRACT
Proteins of the B-cell lymphoma 2 family are crucial for the regulation of apoptosis. B-cell lymphoma 2-associated X is a pro-apoptotic protein, while B-cell lymphoma 2 protein opposes apoptosis. The influence of 1 microM 2-methoxyestradiol was investigated on the expression levels of these two proteins in MCF-7 cells. 2-Methoxyestradiol exposure did not influence B-cell lymphoma 2 protein expression levels after 24 h of exposure. In contrast, B-cell lymphoma 2-associated X protein levels were significantly reduced. An improved differential interference contrasting technique revealed compromised cell density and the presence of a mitotic block in exposed cells. The study proposes that the influence of 2-methoxyestradiol on the expression of these proteins may be time- and cell type dependent and thus not evident d uring the mitotic block observed. Investigation of the regulation of the B-cell lymphoma 2 family will allow researchers to consider signaling pathways for diseases where apoptosis can potentially be controlled.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Protein Biosynthesis
/
Dimethyl Sulfoxide
/
Estradiol
Limits:
Humans
Language:
English
Journal:
Biocell
Journal subject:
Clulas
Year:
2009
Type:
Article
Affiliation country:
South Africa
Institution/Affiliation country:
University of Pretoria/ZA
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