Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells
Biocell
;
33(1): 39-47, Apr. 2009. ilus
Article
in English
| LILACS
| ID: lil-595028
ABSTRACT
Progenitor cells play an important biological role in tooth and bone formation, and previous analyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC-23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber, whereas, MDPC-23 showed odontoblast-like characteristics but with a low ability to induce dentin formation. This study shows that MDPC-23 cells are especial cel ls, which possess morphological and functional characteristics of odontoblast-like cells expressing dentin sialophosphoprotein in vivo. In contrast, dentin sialophosphoprotein gene and protein expression was not detected in both cell lines in vitro. The intra-abdominal diffusion chamber appears as an interesting experimental model for studying phenotypic expression of dental pulp cells in vivo.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Sialoglycoproteins
/
Bone Regeneration
/
Collagen Type I
/
Odontoblasts
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Biocell
Journal subject:
Clulas
Year:
2009
Type:
Article
Affiliation country:
Japan
Institution/Affiliation country:
Okayama University/JP
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