Detección de ADN de parvovirus B19 en donantes de sangre de tres hospitales en Santiago, Chile / Parvovirus B19 among blood donors from three hospitals in Santiago, Chile
Rev. méd. Chile
;
139(2): 143-149, feb. 2011. ilus, tab
Article
in Spanish
| LILACS
| ID: lil-595279
ABSTRACT
Background:
Parvovirus B19 (B19) is associated with a wide range of diseases in humans, whose severity depends on the immunological and hematological status of the host. It is transmitted mainly through the airway but also by transfusions.Aim:
To determine the B19 DNA carrier frequency in a population of volunteer blood donors from three hospitals blood banks in Santiago, Chile, and to determine the viral load in DNA positive cases. Material andMethods:
A total of477 serum samples were analyzed. The screening of B19 DNA was carried out by nested polymerase chain reaction (PCR) directed to the non-structural region of the virus (NS1). The viral load in positives cases was quantified by NS1 Real Time PCR.Results:
Parvovirus B19 was detected in four samples, rendering a frequency of 1119. The viral loads ranged from less than 2000 to 5,626 x 10(5) genome equivalents/ml.Conclusions:
Parvovirus B19 was present in four of 477 blood bank blood donors from three hospitals in Santiago.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Blood Donors
/
DNA, Viral
/
Parvoviridae Infections
Limits:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
Country/Region as subject:
South America
/
Chile
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
2011
Type:
Article
/
Project document
Affiliation country:
Chile
Institution/Affiliation country:
Universidad de Chile/CL
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