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Proteomic analysis of whey from bovine colostrum and mature milk
Golinelli, Luciana Pacheco; Conte-Junior, Carlos Adam; Paschoalin, Vânia Margaret Flosi; Silva, Joab Trajano.
  • Golinelli, Luciana Pacheco; Universidade Federal do Rio de Janeiro. Instituto de Química. Rio de Janeiro. BR
  • Conte-Junior, Carlos Adam; Universidade Federal do Rio de Janeiro. Instituto de Química. Rio de Janeiro. BR
  • Paschoalin, Vânia Margaret Flosi; Universidade Federal do Rio de Janeiro. Instituto de Química. Rio de Janeiro. BR
  • Silva, Joab Trajano; Universidade Federal do Rio de Janeiro. Instituto de Química. Rio de Janeiro. BR
Braz. arch. biol. technol ; 54(4): 761-768, July-Aug. 2011. graf, tab
Article in English | LILACS | ID: lil-595630
ABSTRACT
The aim of this study was to standardize a methodology to obtain two-dimensional (2D) maps of whey proteins from bovine colostrum and mature milk, using two-dimensional electrophoresis, in order to identify the minor proteins by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI TOF/TOF MS). A total of 38 proteins were identified, 20 spots in the colostrum whey and 18 in the mature milk whey; 5 of them were identified for the first time.

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Full text: Available Index: LILACS (Americas) Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2011 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Rio de Janeiro/BR

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Full text: Available Index: LILACS (Americas) Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2011 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Rio de Janeiro/BR