Supraorganized collagen enhances Schwann cell reactivity and organization in vitro
Braz. j. med. biol. res
;
44(7): 682-687, July 2011. ilus, tab
Article
in English
| LILACS
| ID: lil-595697
ABSTRACT
We investigated the reactivity and expression of basal lamina collagen by Schwann cells (SCs) cultivated on a supraorganized bovine-derived collagen substrate. SC cultures were obtained from sciatic nerves of neonatal Sprague-Dawley rats and seeded on 24-well culture plates containing collagen substrate. The homogeneity of the cultures was evaluated with an SC marker antibody (anti-S-100). After 1 week, the cultures were fixed and processed for immunocytochemistry by using antibodies against type IV collagen, S-100 and p75NTR (pan neurotrophin receptor) and for scanning electron microscopy (SEM). Positive labeling with antibodies to the cited molecules was observed, indicating that the collagen substrate stimulates SC alignment and adhesion (collagen IV labeling - organized collagen substrate 706.33 ± 370.86, non-organized collagen substrate 744.00 ± 262.09; S-100 labeling - organized collagen 3809.00 ± 120.28, non-organized collagen 3026.00 ± 144.63, P < 0.05) and reactivity (p75NTR labeling - organized collagen 2156.33 ± 561.78, non-organized collagen 1424.00 ± 405.90, P < 0.05; means ± standard error of the mean in absorbance units). Cell alignment and adhesion to the substrate were confirmed by SEM analysis. The present results indicate that the collagen substrate with an aligned suprastructure, as seen by polarized light microscopy, provides an adequate scaffold for SCs, which in turn may increase the efficiency of the nerve regenerative process after in vivo repair.
Full text:
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Index:
LILACS (Americas)
Main subject:
Schwann Cells
/
Receptors, Nerve Growth Factor
/
Collagen Type IV
/
Extracellular Matrix
/
Nerve Regeneration
Limits:
Animals
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2011
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual de Campinas/BR
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