Antiherpes screening of marine organisms from Colombian Caribbean Sea
Rev. bras. farmacogn
;
21(4): 608-614, jul.-ago. 2011. tab
Article
in English
| LILACS
| ID: lil-596246
ABSTRACT
The exploration of marine environment represents a promising strategy in the search for new active antiviral compounds. The isolation and characterization of the nucleosides spongothymidine and spongouridine from the sponge Cryptotethia crypta used as models for the synthesis of ara-A (vidarabine), that has been used therapeutically against herpetic encephalitis, was the most important contribution since the late 1970s. This paper describes the in vitro antiviral evaluation of 26 organic extracts obtained from eleven octocoral species and fifteen marine sponges. Cytotoxicity was evaluated on Vero cells by MTT assay and the antiviral activity was tested against Herpes Simplex Virus type 1 (HSV-1, KOS strain) by plaque number reduction assay. Results were expressed as 50 percent cytotoxic (CC50) and 50 percent inhibitory (IC50) concentrations, respectively, in order to calculate the selectivity index (SI= CC50/IC50) of each extract. Among the tested marine octocoral species, only three extracts showed antiviral activity, but with low selectivity indices (<3.0). Among the tested marine sponges, eight extracts showed SI values higher than 2.0, and three can be considered promising (Aka cachacrouense, Niphates erecta and Dragmacidon reticulatum) with SI values of 5.0, 8.0 and 11.7, respectively, meriting complementary studies in order to identify the bioactive components of these sponge extracts, which are in course now.
Full text:
Available
Index:
LILACS (Americas)
Type of study:
Diagnostic study
/
Screening study
Country/Region as subject:
South America
/
Colombia
Language:
English
Journal:
Rev. bras. farmacogn
Journal subject:
Pharmacy
Year:
2011
Type:
Article
/
Project document
Affiliation country:
Brazil
/
Colombia
Institution/Affiliation country:
Universidad Nacional de Colombia/CO
/
Universidade Federal de Santa Catarina/BR
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