Molecular techniques for the study and diagnosis of parasite infection
J. venom. anim. toxins incl. trop. dis
;
17(3): 239-248, 2011.
Article
in English
| LILACS
| ID: lil-597222
ABSTRACT
In parasitology, routine laboratory diagnosis involves conventional methods, such as optical microscopy, used for the morphological identification of parasites. Currently, molecular biology techniques are increasingly used to diagnose parasite structures in order to enhance the identification and characterization of parasites. The objective of the present study was to review the main current and new diagnostic techniques for confirmation of parasite infections, namely polymerase chain reaction (PCR), real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), Luminex xMAP, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and restriction fragment length polymorphism (RFLP), in addition to microsatellites. Molecular assays have comprehensively assisted in the diagnosis, treatment and epidemiological studies of parasitic diseases that affect people worldwide, helping to control parasitic disease mortality.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Parasitic Diseases
/
Polymerase Chain Reaction
/
Random Amplified Polymorphic DNA Technique
/
Reverse Transcriptase Polymerase Chain Reaction
/
Molecular Diagnostic Techniques
/
Amplified Fragment Length Polymorphism Analysis
Type of study:
Diagnostic study
/
Prognostic study
Language:
English
Journal:
J. venom. anim. toxins incl. trop. dis
Journal subject:
Toxicology
Year:
2011
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of Rio Grande do Sul/BR
/
Feevale University/BR
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