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Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates
Ribeiro, Ricardo Souza; Ladeira, Luisa; Rezende, Antonio Mauro; Fontes, Cor Jesus Fernandes; Carvalho, Luzia Helena; Brito, Cristiana Ferreira Alves de.
  • Ribeiro, Ricardo Souza; Laboratório de Malária.
  • Ladeira, Luisa; Laboratório de Malária.
  • Rezende, Antonio Mauro; Fiocruz. Instituto de Pesquisa René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte. BR
  • Fontes, Cor Jesus Fernandes; Universidade Federal do Mato Grosso. Departamento de Clínica Médica. Cuiabá. BR
  • Carvalho, Luzia Helena; Laboratório de Malária.
  • Brito, Cristiana Ferreira Alves de; Laboratório de Malária.
Mem. Inst. Oswaldo Cruz ; 106(supl.1): 27-33, Aug. 2011. ilus, graf, tab
Article in English | LILACS | ID: lil-597241
ABSTRACT
Reliable molecular markers are essential for a better understanding of the molecular epidemiology of Plasmodium vivax, which is a neglected human malaria parasite. The aim of this study was to analyze the genetic diversity of P. vivax isolates from the Brazilian Amazon using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the highly polymorphic merozoite surface protein-3alpha (PvMSP-3α) gene. To accomplish this, 60 isolates of P. vivax from different endemic areas in the Brazilian Amazon were collected. The PvMSP-3α gene was amplified by nested-PCR. Three major types of the PvMSP-3α locus were detected at different frequencies type A (68 percent), B (15 percent) and C (17 percent). A single sample showed two PCR fragments, which corresponded to infection with types A and C. PCR-RFLP analysis using the HhaI restriction enzyme for 52 isolates clearly identified 11 haplotypes, eight of which were from type A, two from type B and only one from type C. Seven other isolates did not show a clear pattern using PCR-RFLP. This result might be due to multiple clone infections. This study showed a high diversity of the PvMSP-3α gene among P. vivax isolates from the Brazilian Amazon, but also indicated that the detection performance of PCR-RFLP of the PvMSP-3α gene may not be sufficient to detect multiple clone infections.
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Full text: Available Index: LILACS (Americas) Main subject: Plasmodium vivax / Genetic Variation / Protozoan Proteins / Antigens, Protozoan Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2011 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Fiocruz/BR / Universidade Federal do Mato Grosso/BR

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Full text: Available Index: LILACS (Americas) Main subject: Plasmodium vivax / Genetic Variation / Protozoan Proteins / Antigens, Protozoan Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2011 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Fiocruz/BR / Universidade Federal do Mato Grosso/BR